Bjorling D E, Saban M R, Zine M J, Haak-Frendscho M, Graziano F M, Saban R
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
J Urol. 1994 Nov;152(5 Pt 1):1603-8. doi: 10.1016/s0022-5347(17)32487-4.
Studies of human bladder inflammation have been limited to examination of urine, bladder biopsy, or examination of autopsy material. We have developed an in vitro bladder passive sensitization technique which can measure type I responses of isolated human bladder tissue. We have compared these results using human tissue to those obtained with bladder tissue from guinea pigs and Rhesus monkeys. In our studies, bladder tissue was passively sensitized in vitro for 20 hours with immunoglobulin-containing serum. Subsequent antigen challenge of the passively sensitized tissue resulted in a time-dependent contraction that was accompanied by tissue histamine release. Contractions of guinea pig, monkey and human bladder tissue reached 79%, 100% and 78% of the maximal contraction induced by potassium chloride. In contrast, adjacent strips of unsensitized tissue had no detectable response to antigen challenge. The responses were reduced in the presence of histamine H1 receptor blockade with pyrilamine and abolished in the presence of a concomitant blockade of leukotriene synthesis with nordihydroguaiaretic acid (NDGA). Blockade of cyclooxygenase activity with indomethacin increased the contraction of the sensitized guinea pig bladder in response to antigen challenge. These findings demonstrate that in vitro passive sensitization of human bladder tissue can be used to investigate basic mechanisms of noninfectious bladder inflammation in humans.
对人类膀胱炎症的研究一直局限于尿液检查、膀胱活检或尸检材料检查。我们开发了一种体外膀胱被动致敏技术，该技术可以测量分离的人类膀胱组织的I型反应。我们将使用人体组织得到的这些结果与用豚鼠和恒河猴的膀胱组织得到的结果进行了比较。在我们的研究中，膀胱组织在体外用含免疫球蛋白的血清被动致敏20小时。随后对被动致敏组织进行抗原激发，导致了时间依赖性收缩，并伴有组织组胺释放。豚鼠、猴子和人类膀胱组织的收缩分别达到氯化钾诱导的最大收缩的79%、100%和78%。相比之下，相邻的未致敏组织条对抗原激发没有可检测到的反应。在存在扑尔敏阻断组胺H1受体的情况下，反应减弱；在同时存在去甲二氢愈创木酸（NDGA）阻断白三烯合成的情况下，反应消失。用消炎痛阻断环氧化酶活性会增加致敏豚鼠膀胱对抗原激发的收缩。这些发现表明，人类膀胱组织的体外被动致敏可用于研究人类非感染性膀胱炎症的基本机制。
