Kim Y S, Levin R M, Wein A J, Longhurst P A
Division of Urology, University of Pennsylvania School of Medicine, Philadelphia.
J Urol. 1992 Jan;147(1):270-3. doi: 10.1016/s0022-5347(17)37211-7.
Permeability of the guinea pig urinary bladder was investigated in a model of experimental cystitis induced by intravesical antigen challenge following sensitization. Guinea pigs were sensitized by intraperitoneal injections of ovalbumin (10 mg./kg.) given on days 1, 3, and 5. The studies described were done four weeks after the last injection. Controls (injected with saline) were used at the same time as sensitized animals. Each group (control and sensitized), was divided into two subgroups; guinea pigs challenged with intravesical ovalbumin (10 mg./ml., one ml.) and those receiving one ml. saline intravesically. Immediately following the antigen (or saline) challenge, one ml. of 14C-urea urea was placed into the bladder for two hours. We examined the peripheral blood concentrations of 14C-urea at periods of time up to 120 minutes. There was a progressive increase in the blood level of 14C-urea with time only in the sensitized group challenged with antigen (ovalbumin). There was no 14C-urea present in the blood of the sensitized group without antigen challenge, or in either unsensitized group. We also measured isotope concentration in the bladders and found a significantly higher concentration of isotope in the bladders from ovalbumin-treated sensitized guinea pigs. We believe that this animal model of cystitis is an improvement over previous models because of its physiological relevance. In this model, cystitis is produced without mechanical or chemical damage to the bladder mucosa. A discussion of the model in relation to interstitial cystitis is presented.
在致敏后通过膀胱内抗原激发诱导的实验性膀胱炎模型中，研究了豚鼠膀胱的通透性。豚鼠在第1、3和5天通过腹腔注射卵清蛋白（10mg/kg）进行致敏。所述研究在最后一次注射四周后进行。对照组（注射生理盐水）与致敏动物同时使用。每组（对照组和致敏组）分为两个亚组；用膀胱内卵清蛋白（10mg/ml，1ml）激发的豚鼠和膀胱内接受1ml生理盐水的豚鼠。在抗原（或生理盐水）激发后，立即将1ml的14C-尿素放入膀胱中两小时。我们在长达120分钟的时间段内检测外周血中14C-尿素的浓度。仅在接受抗原（卵清蛋白）激发的致敏组中，14C-尿素的血药浓度随时间逐渐升高。未接受抗原激发的致敏组或任何未致敏组的血液中均未检测到14C-尿素。我们还测量了膀胱中的同位素浓度，发现经卵清蛋白处理的致敏豚鼠膀胱中的同位素浓度明显更高。我们认为，这种膀胱炎动物模型由于其生理相关性，较之前的模型有所改进。在该模型中，膀胱炎的产生未对膀胱黏膜造成机械或化学损伤。本文还讨论了该模型与间质性膀胱炎的关系。
