Emoto N, Isozaki O, Ohmura E, Ito F, Tsushima T, Shizume K, Demura H, Toma H
Department of Medicine, Tokyo Women's Medical College, Japan.
J Urol. 1994 Nov;152(5 Pt 1):1626-31. doi: 10.1016/s0022-5347(17)32492-8.
To investigate the role of basic fibroblast growth factor (FGF) in renal cell carcinoma growth, we have analyzed the expression of mRNA of basic FGF. In 7 of 15 cases, basic FGF mRNA level in renal cell carcinoma tissues was higher than that in corresponding normal tissues. However, the tumor-to-normal ratios of expression levels are chiefly less than 2.0 and, in 5 cases, are even less than 1.0. Furthermore, there was no correlation between the ratio and the clinical stage. In protein analysis, we could not find any difference between basic FGF extracted from renal cell carcinomas and that from normal kidney tissues in bioactivity, immunoreactivity, molecular weight and affinity to heparin. On the other hand, anti-basic FGF monoclonal antibody inhibited the growth of a renal cell carcinoma cell line, VMRC-RCW, and this inhibition was reversed by an extraphysiological amount of exogenous basic FGF (100 ng./ml.). These results suggest that basic FGF itself may have no pivotal role in renal cell carcinoma etiology but is involved in the growth of renal cell carcinomas in an autocrine manner.
为研究碱性成纤维细胞生长因子（FGF）在肾细胞癌生长中的作用，我们分析了碱性FGF的mRNA表达。在15例中的7例中，肾细胞癌组织中碱性FGF mRNA水平高于相应的正常组织。然而，表达水平的肿瘤与正常组织比值主要小于2.0，且在5例中甚至小于1.0。此外，该比值与临床分期之间无相关性。在蛋白质分析中，我们未发现从肾细胞癌中提取的碱性FGF与从正常肾组织中提取的碱性FGF在生物活性、免疫反应性、分子量及与肝素的亲和力方面存在任何差异。另一方面，抗碱性FGF单克隆抗体抑制了肾癌细胞系VMRC-RCW的生长，且这种抑制作用可被超生理量的外源性碱性FGF（100 ng/ml）逆转。这些结果表明，碱性FGF本身在肾细胞癌病因学中可能无关键作用，但以自分泌方式参与肾细胞癌的生长。
