Takahashi J A, Mori H, Fukumoto M, Igarashi K, Jaye M, Oda Y, Kikuchi H, Hatanaka M
Department of Molecular Virology, Faculty of Medicine, Kyoto University, Japan.
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5710-4. doi: 10.1073/pnas.87.15.5710.
The growth autonomy of human tumor cells is considered due to the endogenous production of growth factors. Transcriptional expression of candidates for autocrine stimulatory factors such as basic fibroblast growth factor (FGF), acidic FGF, and transforming growth factor type beta were determined in human brain tumors. Basic FGF was expressed abundantly in 17 of 18 gliomas, 20 of 22 meninglomas, and 0 of 5 metastatic brain tumors. The level of mRNA expression of acidic FGF in gliomas was significant. In contrast, transforming growth factor type beta 1 was expressed in all the samples investigated. The mRNA for basic FGF and its peptide were localized in tumor cells in vivo by in situ hybridization and immunohistochemistry, showing that basic FGF is actually produced in tumor cells. Our results suggest that tumor-derived basic FGF is involved in the progression of gliomas and meningiomas in vivo, whereas acidic FGF is expressed in a tumor origin-specific manner, suggesting that acidic FGF works in tandem with basic FGF in glioma tumorigenesis.
人类肿瘤细胞的生长自主性被认为是由于生长因子的内源性产生。在人脑肿瘤中测定了自分泌刺激因子候选物如碱性成纤维细胞生长因子（FGF）、酸性FGF和转化生长因子β型的转录表达。碱性FGF在18例胶质瘤中的17例、22例脑膜瘤中的20例以及5例转移性脑肿瘤中的0例中大量表达。胶质瘤中酸性FGF的mRNA表达水平显著。相比之下，转化生长因子β1在所有研究样本中均有表达。通过原位杂交和免疫组织化学在体内将碱性FGF的mRNA及其肽定位在肿瘤细胞中，表明碱性FGF实际上是在肿瘤细胞中产生的。我们的结果表明，肿瘤衍生的碱性FGF参与了体内胶质瘤和脑膜瘤的进展，而酸性FGF以肿瘤起源特异性方式表达，表明酸性FGF在胶质瘤肿瘤发生中与碱性FGF协同作用。
