Chen S Q, Xue K X, Ma G J, Wu J Z, Wang H, Xiang L P, Cheng N
Cytogenetic Laboratory, Jiangsu Institute of Cancer Research, Nanjing, People's Republic of China.
Mutat Res. 1994 Oct 1;310(1):113-6. doi: 10.1016/0027-5107(94)90014-0.
The genotoxicity of human fetal cell extract (HFCE) and its effect on the frequency of micronucleated polychromatic erythrocytes (PCE-MNF) in mice induced by cyclophosphamide (CP) were studied. Statistically significant differences were not found between the control group and each group treated with HFCE (0.3, 3, 30 mg/kg bw). CP (200 mg/kg bw) induced a marked increase in MNF (P < 0.01). Administered together with CP, HFCE suppressed the increase of MNF induced by CP. The reduction effect is dependent on the dose of HFCE. At doses of 3 and 30 mg/kg bw HFCE, MNF decreased markedly (P < 0.05 and < 0.01, respectively). It showed that HFCE did not induce micronucleus formation, while it could suppress the micronucleus formation induced by CP in mice. The results suggested that HFCE might be antimutagenic and have potential value in clinical application.
研究了人胎儿细胞提取物（HFCE）的遗传毒性及其对环磷酰胺（CP）诱导的小鼠微核多染红细胞频率（PCE-MNF）的影响。对照组与各剂量HFCE（0.3、3、30mg/kg体重）处理组之间未发现统计学上的显著差异。CP（200mg/kg体重）诱导微核频率显著增加（P<0.01）。与CP联合给药时，HFCE抑制了CP诱导的微核频率增加。这种降低作用取决于HFCE的剂量。在3和30mg/kg体重的HFCE剂量下，微核频率显著降低（分别为P<0.05和<0.01）。结果表明，HFCE不会诱导微核形成，而能抑制CP诱导的小鼠微核形成。结果提示，HFCE可能具有抗诱变作用，在临床应用中具有潜在价值。
