Sato S, Fujimoto M, Ihn H, Kikuchi K, Takehara K
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Arch Dermatol. 1994 Oct;130(10):1273-7.
Recently, we detected antihistone antibodies (AHAs) in patients with localized scleroderma. However, the exact antigen specificity of AHAs in this disease is still unknown. Therefore, we determined the reactivity of AHAs with five individual histones and the correlation of AHAs with rheumatoid factor in localized scleroderma by means of enzyme-linked immunosorbent assay. Twenty patients with localized scleroderma who had IgG and/or IgM AHAs, as determined by enzyme-linked immunosorbent assay, were examined. These patients were classified into the following three subgroups: patients with generalized morphea (n = 11), patients with linear scleroderma (n = 6), and patients with morphea (n = 3).
In generalized morphea, IgG AHAs strongly reacted with histones H1, H2A, and H2B; and IgM AHAs strongly reacted with H1 and H2B, as determined by means of enzyme-linked immunosorbent assay. The pattern of reactivity in linear scleroderma and morphea was similar to that in generalized morphea. A homogeneous immunofluorescent pattern on HEp-2 cells, which was produced by localized scleroderma sera, was completely abolished by absorption with total histones. By employing a latex agglutination test, IgM rheumatoid factor was detected in 60% of the 20 patients with localized scleroderma and at a frequency of 82% in those with generalized morphea. However, an absorption test of rheumatoid factor activity with human IgG revealed no cross-reactivity of AHAs with rheumatoid factor.
Our data suggest that AHAs in localized scleroderma are directed against native chromatin, since H1, H2A, and H2B occupy a relatively exposed portion of chromatin.
最近，我们在局限性硬皮病患者中检测到抗组蛋白抗体（AHA）。然而，该疾病中AHA的确切抗原特异性仍不清楚。因此，我们通过酶联免疫吸附测定法确定了局限性硬皮病中AHA与五种单个组蛋白的反应性以及AHA与类风湿因子的相关性。对20例经酶联免疫吸附测定法确定存在IgG和/或IgM AHA的局限性硬皮病患者进行了检查。这些患者被分为以下三个亚组：泛发性硬斑病患者（n = 11）、线状硬皮病患者（n = 6）和硬斑病患者（n = 3）。
通过酶联免疫吸附测定法确定，在泛发性硬斑病中，IgG AHA与组蛋白H1、H2A和H2B强烈反应；IgM AHA与H1和H2B强烈反应。线状硬皮病和硬斑病中的反应模式与泛发性硬斑病相似。局限性硬皮病血清在HEp-2细胞上产生的均匀免疫荧光模式，在用总组蛋白吸收后完全消失。通过乳胶凝集试验，在20例局限性硬皮病患者中有60%检测到IgM类风湿因子，在泛发性硬斑病患者中的检测频率为82%。然而，用人IgG对类风湿因子活性进行吸收试验显示，AHA与类风湿因子无交叉反应。
我们的数据表明，局限性硬皮病中的AHA针对的是天然染色质，因为H1、H2A和H2B占据了染色质相对暴露的部分。
