抗核小体抗体是局限性硬皮病中的一种主要自身抗体。

Antinucleosome antibody is a major autoantibody in localized scleroderma.

作者信息

Sato S, Kodera M, Hasegawa M, Fujimoto M, Takehara K

机构信息

Department of Dermatology, Kanazawa University Graduate School of Medical Science, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8641, Japan.

出版信息

Br J Dermatol. 2004 Dec;151(6):1182-8. doi: 10.1111/j.1365-2133.2004.06256.x.

DOI:10.1111/j.1365-2133.2004.06256.x

PMID:15606513

Abstract

BACKGROUND

Localized scleroderma (LSc) exhibits autoimmunity, and antihistone antibody is frequently detected. The major antigens recognized by antihistone antibody are histones H1, H2A and H2B, which are located on the outer side of the nucleosome and are relatively more accessible for antibody binding. Therefore, it has been hypothesized that antihistone antibody is induced by nucleosome or native chromatin as immunogens in LSc.

OBJECTIVES

To determine whether antinucleosome antibody is present in patients with LSc.

METHODS

Antinucleosome antibody, antihistone antibody and antidouble-stranded DNA (dsDNA) antibody were determined by enzyme-linked immunosorbent assay. Results IgG or IgM antinucleosome antibody was detected more frequently in patients with LSc than was antihistone antibody: in 40 of 49 (82%) vs. 26 of 49 (53%), respectively. No patients had anti-dsDNA antibody. The prevalence of antinucleosome antibody positivity was comparable in the three subgroups of LSc (generalized morphoea, 89%; linear scleroderma, 71%; morphoea, 90%). Patients with systemic lupus erythematosus (SLE) exhibited a similar frequency of antinucleosome antibody positivity (13 of 15, 87%), but their IgG levels of this autoantibody were much higher than those found in patients with LSc. By contrast, IgM antinucleosome antibody levels were normal in patients with SLE, while they were significantly increased in patients with LSc compared with normal controls. Antinucleosome antibody was also detected at lower frequency in patients with systemic sclerosis (five of 20, 25%) or dermatomyositis (five of 15, 33%). Nucleosome-restricted antibodies, i.e. antibodies that react with the whole nucleosome particle but not with its individual components (histones and dsDNA) were also present in 35% of patients with LSc.

CONCLUSIONS

Although antinucleosome antibody was not specific to LSc, its high prevalence in LSc indicates that antinucleosome antibody is a major autoantibody in this disease.

摘要

背景

局限性硬皮病（LSc）表现出自身免疫性，且经常检测到抗组蛋白抗体。抗组蛋白抗体识别的主要抗原是组蛋白H1、H2A和H2B，它们位于核小体外侧，相对更容易与抗体结合。因此，有人推测在局限性硬皮病中，抗组蛋白抗体是由核小体或天然染色质作为免疫原诱导产生的。

目的

确定局限性硬皮病患者是否存在抗核小体抗体。

方法

采用酶联免疫吸附测定法检测抗核小体抗体、抗组蛋白抗体和抗双链DNA（dsDNA）抗体。结果：局限性硬皮病患者中检测到IgG或IgM抗核小体抗体的频率高于抗组蛋白抗体，分别为49例中的40例（82%）和49例中的26例（53%）。无患者有抗dsDNA抗体。局限性硬皮病的三个亚组（泛发性硬斑病，89%；线状硬皮病，71%；硬斑病，90%）中抗核小体抗体阳性率相当。系统性红斑狼疮（SLE）患者抗核小体抗体阳性频率相似（15例中的13例，87%），但其该自身抗体的IgG水平远高于局限性硬皮病患者。相比之下，SLE患者的IgM抗核小体抗体水平正常，而局限性硬皮病患者与正常对照相比显著升高（P<0.05）。系统性硬化症患者（20例中的5例，25%）或皮肌炎患者（15例中的5例，33%）中抗核小体抗体检测频率也较低。35%的局限性硬皮病患者还存在核小体限制性抗体，即与整个核小体颗粒反应但不与其单个成分（组蛋白和dsDNA）反应的抗体。