Wojcik E M, Katz R L, Fanning T V, Johnston D A
Department of Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Anal Quant Cytol Histol. 1994 Aug;16(4):287-92.
Mantle cell lymphoma (MCL) is a distinct type of non-Hodgkin's lymphoma, which, based on the architectural pattern, has been divided into mantle zone (MCL-MZ), allegedly associated with longer survival, and diffuse (MCL-D) subtypes. To determine whether there are morphonuclear differences between MCL-MZ and MCL-D, 37 fine needle aspirates (FNAs) obtained from 32 patients with MCL (21 cases of MCL-MZ and 11 of MCL-D) were evaluated using digital cell image analysis. Twenty-one morphometric parameters related to geometry, color, texture and densitometry were evaluated on 100 cells from each case. Aspirated material was stained with either the Diff-Quik or Feulgen method. Statistical analysis (student's t test and nonparametric Mann-Whitney test) was performed. No significant differences between MCL-MZ and MCL-D were found in any of the 21 studied morphonuclear parameters. The morphometric analysis results were similar using the Diff-Quik and Feulgen techniques. This study confirmed the overlapping cytologic spectrum of MCL-MZ and MCL-D and indicated that histologic specimens demonstrating a specific architectural pattern are necessary to differentiate these two variants of MCL. Either Diff-Quik- or Feulgen-stained tissues may be used for morphonuclear measurements in FNAs of MCL since both give similar results.
套细胞淋巴瘤（MCL）是一种独特类型的非霍奇金淋巴瘤，根据其结构模式，已被分为套区型（MCL-MZ，据称生存期较长）和弥漫型（MCL-D）亚型。为了确定MCL-MZ和MCL-D之间是否存在形态核差异，使用数字细胞图像分析对从32例MCL患者（21例MCL-MZ和11例MCL-D）获取的37份细针穿刺抽吸物（FNA）进行了评估。对每个病例的100个细胞评估了21个与几何形状、颜色、纹理和密度测定相关的形态测量参数。抽吸物用Diff-Quik或福尔根染色法染色。进行了统计分析（学生t检验和非参数曼-惠特尼检验）。在研究的21个形态核参数中的任何一个中，MCL-MZ和MCL-D之间均未发现显著差异。使用Diff-Quik和福尔根技术的形态测量分析结果相似。本研究证实了MCL-MZ和MCL-D在细胞学谱上的重叠，并表明需要组织学标本显示特定的结构模式才能区分这两种MCL变体。由于两者结果相似，Diff-Quik染色或福尔根染色的组织均可用于MCL的FNA中的形态核测量。
