Karperien M, van Dijk T B, Hoeijmakers T, Cremers F, Abou-Samra A B, Boonstra J, de Laat S W, Defize L H
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Mech Dev. 1994 Jul;47(1):29-42. doi: 10.1016/0925-4773(94)90093-0.
In this paper we describe the cloning of the mouse Parathyroid Hormone/Parathyroid Hormone related Peptide Receptor (PTH/PTHrPR) cDNA and expression of its mRNA during mouse postimplantation development from day 5.5 until day 15.5 post coitum (p.c.). In support of a model from previous studies, in which parietal endoderm differentiation is regulated by the interaction of the PTH/PTHrPR and Parathyroid Hormone related Peptide (PTHrP), high levels of PTH/PTHrPR mRNA levels were detected in developing parietal endoderm from day 5.5 p.c. and onwards. In the embryo proper, PTH/PTHrPR mRNA expression was mainly detected at sites of epithelium/mesenchyme interactions, starting at day 9.5 p.c. in the epithelium of the intestine and later in the mesenchyme of the lung, the epithelium of meso- and metanephric tubuli, the dermis and at all sites where bone formation takes place. The complexity of the PTH/PTHrPR expression pattern suggests tight developmental regulation and indicates multiple roles in embryogenesis for the receptor and its ligands, not only in extraembryonic tissue but also in the formation of various organs.
在本文中，我们描述了小鼠甲状旁腺激素/甲状旁腺激素相关肽受体（PTH/PTHrPR）cDNA的克隆及其mRNA在小鼠植入后从妊娠第5.5天到第15.5天发育过程中的表达情况。此前的研究提出了一个模型，即壁内胚层分化受PTH/PTHrPR与甲状旁腺激素相关肽（PTHrP）相互作用的调控，支持这一模型的是，从妊娠第5.5天起，在发育中的壁内胚层中检测到高水平的PTH/PTHrPR mRNA。在胚胎本身，PTH/PTHrPR mRNA表达主要在上皮/间充质相互作用的部位检测到，从妊娠第9.5天开始在肠上皮中出现，随后在肺间充质、中肾小管和后肾小管上皮、真皮以及所有发生骨形成的部位出现。PTH/PTHrPR表达模式的复杂性表明其受到严格的发育调控，并暗示该受体及其配体在胚胎发生中具有多种作用，不仅在胚外组织中，而且在各种器官的形成中都发挥作用。
