Fernández-Casalderrey A, Ferrando M D, Andreu-Moliner E
Department of Animal Biology (Animal Physiology), Faculty of Biological Sciences, University of Valencia, Spain.
Ecotoxicol Environ Saf. 1994 Feb;27(1):82-9. doi: 10.1006/eesa.1994.1008.
Daphnia magna was exposed to sublethal levels (1/4, 1/2, and 2/3 LC50 and LC50) of endosulfan and diazinon to determine the effect of these pesticides on filtration and ingestion rates. The experiments were performed with the unicellular algae Nannochloris oculata in a density of 5 x 10(5) cells/ml. Prior to these experiments, the acute toxicity of both pesticides was evaluated to calculate the LC50s. The 24-hr LC50 values were 0.62 mg/liter and 0.9 microgram/liter for endosulfan and diazinon, respectively. Rates of filtration and ingestion declined with increasing toxicant concentrations after a short exposure of 5 hr. The effective concentration at which feeding rates were reduced to 50% of those in controls (EC50) was calculated for both pesticides. These values were 0.44 and 0.61 mg/liter for filtration and ingestion rates, respectively, in the case of endosulfan and 0.47 and 0.60 microgram/liter, respectively, in the case of diazinon.
将大型溞暴露于硫丹和二嗪农的亚致死水平(1/4、1/2、2/3 LC50和LC50),以确定这些农药对滤过率和摄食率的影响。实验使用密度为5×10⁵个细胞/毫升的单细胞藻类眼点拟微绿球藻进行。在这些实验之前,评估了两种农药的急性毒性以计算LC50。硫丹和二嗪农的24小时LC50值分别为0.62毫克/升和0.9微克/升。经过5小时的短时间暴露后,滤过率和摄食率随毒物浓度增加而下降。计算了两种农药使摄食率降低至对照摄食率50%时的有效浓度(EC50)。对于硫丹,滤过率和摄食率的这些值分别为0.44毫克/升和0.61毫克/升,对于二嗪农,分别为0.47微克/升和0.60微克/升。