Janssen C R, Ferrando M D, Persoone G
Laboratory for Biological Research in Aquatic Pollution, University of Ghent, Belgium.
Ecotoxicol Environ Saf. 1994 Aug;28(3):244-55. doi: 10.1006/eesa.1994.1050.
The swimming behavior of the freshwater rotifer Brachionus calyciflorus exposed to copper (Cu), pentachlorophenol (PCP), 3,4-dichloroaniline (DCA), and lindane, for periods ranging from 5 min to 5 hr, was examined. A swimming behavior test is described which is based on the rotifers' movement rates as they swim over a grid. For all four toxicants a clear dose-response was observed, with the swimming activity decreasing with increasing toxicant concentrations. For Cu the EC50's, the concentration that reduced the swimming activity to 50% of that of the control value, sharply decreased from 0.22 mg/liter after an exposure of 5 min to 0.068, 0.038, and 0.014 mg/liter after exposures of 30, 60, and 300 min, respectively. PCP affected the rotifers' swimming behavior more gradually, with EC50's decreasing from 7.0 mg/liter after an exposure of 5 min to 5.9, 5.4, and 1.5 mg/liter after 30, 60, and 300 min, respectively. A similar pattern was found for DCA with EC50's ranging from 193 to 45.5 mg/liter for the 5-min and 3-hr exposures, respectively. Exposed to lindane however, B. calyciflorus swimming activity exhibited a different response, and the EC50's gradually increased from 13.7 mg/liter after an exposure of 5 min to significantly higher values of 16.4 and 18.5 mg/liter after periods of 1 and 5 hr, respectively. The results of the swimming activity assays were compared to those of acute and chronic toxicity tests performed with the same test species. The potential use and relevance of this behavioral test criterion were evaluated and discussed.
研究了淡水轮虫萼花臂尾轮虫（Brachionus calyciflorus）在5分钟至5小时的时间段内暴露于铜（Cu）、五氯苯酚（PCP）、3,4-二氯苯胺（DCA）和林丹后的游泳行为。描述了一种基于轮虫在网格上游泳时的运动速率的游泳行为测试。对于所有四种有毒物质，均观察到明显的剂量反应，游泳活动随有毒物质浓度的增加而降低。对于铜，将游泳活动降低至对照值50%的EC50（半数有效浓度）在暴露5分钟后为0.22毫克/升，在暴露30、60和300分钟后分别急剧降至0.068、0.038和0.014毫克/升。五氯苯酚对轮虫游泳行为的影响较为缓慢，EC50在暴露5分钟后为7.0毫克/升，在30、60和300分钟后分别降至5.9、5.4和1.5毫克/升。3,4-二氯苯胺也有类似模式，5分钟和3小时暴露的EC50分别为193至45.5毫克/升。然而，暴露于林丹后，萼花臂尾轮虫的游泳活动表现出不同的反应，EC50从暴露5分钟后的13.7毫克/升逐渐增加，在暴露1小时和5小时后分别显著升至16.4和18.5毫克/升。将游泳活动测定结果与使用相同测试物种进行的急性和慢性毒性测试结果进行了比较。对该行为测试标准的潜在用途和相关性进行了评估和讨论。
