Dept. of Chemical Ecology and Ecotoxicology, Lund University, Ecology Building, S-223 62, Lund, Sweden.
Environ Sci Pollut Res Int. 1995 Jul;2(1):15-23. doi: 10.1007/BF02987506.
An impedance conversion technique was used to study the behaviour ofGammarus pulex (L.) exposed to acutely toxic concentrations of Pb (0.01, 0.05, 0.1 and 0.5 mg Pb l(-1)) and to field concentrations of Cu ( ≤ 0.05 mg Cu l(-1)). Initial stress responses were studied during short-term exposure (1 h) and sublethal toxic effects were monitored during 7 (Pb) and 35 days (Cu), respectively.Exposure to Pb caused 30 % mortality and resulted in a bioconcentration factor (BCF) of 2700 at 0.5 mg Pb l(-1) after 168 h. Exposure to Cu polluted stream water caused no mortality within 35 days and uptake was low (BCF 5.8).Gammarus pulex reacted with initial stress responses to metal exposure within 30 min. (Cu) or 1 h (Pb). The reactions consisted of increased ventilation and decreased locomotion.Sublethal concentrations of Pb and Cu caused toxic effects on the behaviour of G.pulex after several days of exposure, consisting of increased ventilation and decreased locomotion.Impedance conversion is an appropriate method for detecting stress responses to metals and can be used in "early warning" biomonitoring systems as well as for acute and chronic behavioural toxicity testing.
采用阻抗转换技术研究了铜（≤0.05mgCuL（-1））和野外浓度的铜（≤0.05mgCuL（-1））暴露下，对铜具有急性毒性浓度的铜（0.01、0.05、0.1 和 0.5mgPbL（-1））暴露下，食蚊鱼（Gammaruspulex（L.））的行为。在短期暴露（1h）期间研究了初始应激反应，分别在 7 天（Pb）和 35 天（Cu）期间监测了亚致死毒性效应。暴露于 Pb 导致 30%的死亡率，并在 168h 后导致 0.5mgPbL（-1）时的生物浓缩因子（BCF）为 2700。暴露于受 Cu 污染的溪流水中，在 35 天内没有导致死亡，且吸收量较低（BCF5.8）。食蚊鱼对金属暴露的初始应激反应在 30min（Cu）或 1h（Pb）内作出反应。这些反应包括增加通气和减少运动。在暴露数天后，亚致死浓度的 Pb 和 Cu 对食蚊鱼的行为产生了毒性效应，包括增加通气和减少运动。阻抗转换是一种检测金属应激反应的合适方法，可用于“早期预警”生物监测系统以及急性和慢性行为毒性测试。
