Jokhi P P, King A, Sharkey A M, Smith S K, Loke Y W
Department of Pathology, University of Cambridge, United Kingdom.
J Immunol. 1994 Nov 15;153(10):4427-35.
At the time of human embryo implantation, large numbers of maternal CD56brightCD16- NK cells appear in the uterus. These unusual lymphocytes are believed to control the migration and differentiation of highly invasive fetally derived trophoblast cells, which infiltrate into the maternal uterus to remodel the spiral arteries during the first trimester. One possible mechanism of control is by cytokine production. In this study, highly purified (> 99%) populations of first trimester decidual CD56brightCD16- NK cells and CD3+ T lymphocytes were obtained by using a FACS. These cells were examined by reverse transcriptase PCR for their expression of mRNAs for the following cytokines: granulocyte-macrophage (GM)-CSF, CSF-1, TNF-alpha, IFN-gamma, TGF-beta 1, leukemia-inhibitory factor (LIF), and IL-2. Then, the expression was compared with that of resting PBL. The identity of the PCR products was verified by Southern blotting and hybridization with cytokine-specific probes. Both decidual CD56brightCD16- NK cells and CD3+ T cells were found to express mRNA for CSF-1, TNF-alpha, IFN-gamma TGF-beta 1, and LIF, but GM-CSF mRNA was detected only in CD56bright NK cells. IL-2 mRNA was detected in only some decidual T cell samples, and then only after at least two rounds of amplification. In contrast, peripheral blood CD56brightCD16- NK cells, CD56dimCD16+ NK cells, and CD3+ T cells expressed mRNA only for TNF-alpha and TGF-beta 1, but not for GM-CSF, CSF-1, IFN-gamma, LIF, or IL-2. These results suggest that both decidual NK cells and decidual T cells produce a variety of cytokines that may be involved in the control of trophoblast migration and differentiation during pregnancy.
在人类胚胎着床时，大量母体CD56brightCD16-自然杀伤（NK）细胞出现在子宫中。这些特殊的淋巴细胞被认为可控制具有高度侵袭性的胎儿来源的滋养层细胞的迁移和分化，这些滋养层细胞在妊娠早期浸润到母体子宫中以重塑螺旋动脉。一种可能的控制机制是通过细胞因子的产生。在本研究中，使用荧光激活细胞分选仪（FACS）获得了高度纯化（>99%）的妊娠早期蜕膜CD56brightCD16- NK细胞和CD3+ T淋巴细胞群体。通过逆转录聚合酶链反应（RT-PCR）检测这些细胞中以下细胞因子的mRNA表达：粒细胞-巨噬细胞（GM）-集落刺激因子（CSF）、CSF-1、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、转化生长因子-β1（TGF-β1）、白血病抑制因子（LIF）和白细胞介素-2（IL-2）。然后，将该表达与静息外周血淋巴细胞（PBL）的表达进行比较。通过Southern印迹法以及与细胞因子特异性探针杂交来验证PCR产物的同一性。发现蜕膜CD56brightCD16- NK细胞和CD3+ T细胞均表达CSF-1、TNF-α、IFN-γ、TGF-β1和LIF的mRNA，但GM-CSF mRNA仅在CD56bright NK细胞中检测到。IL-2 mRNA仅在一些蜕膜T细胞样本中检测到，并且仅在至少两轮扩增后才能检测到。相比之下，外周血CD56brightCD16- NK细胞、CD56dimCD16+ NK细胞和CD3+ T细胞仅表达TNF-α和TGF-β1的mRNA，而不表达GM-CSF、CSF-1、IFN-γ、LIF或IL-2的mRNA。这些结果表明，蜕膜NK细胞和蜕膜T细胞均产生多种可能参与妊娠期间滋养层细胞迁移和分化控制的细胞因子。
