Saito S, Kasahara T, Sakakura S, Enomoto M, Umekage H, Harada N, Morii T, Nishikawa K, Narita N, Ichijo M
Department of Obstetrics and Gynecology, Nara Medical University, Japan.
Biochem Biophys Res Commun. 1994 Apr 15;200(1):378-83. doi: 10.1006/bbrc.1994.1459.
Decidual CD16-CD56bright natural killer (NK) cells were sorted from the decidual mononuclear cells (MNC) at the early pregnancy using a fluorescence activated cell sortor. The CD16-CD56bright NK cell population occupies a major population in the decidual MNC, in contrast to a very small population (< 1%) in the peripheral blood MNC. These decidual CD16-CD56bright NK cells produced a large amount of IL-8, i. e., mean of 96.7 +/- 19.8 ng/ml in the 24 hr-cultured supernatants without any stimulant, which was comparable to the IL-8 production by LPS-stimulated peripheral blood MNC. Most of the IL-8 was ascribable to the production from decidual CD16-CD56bright NK cells. Intracytoplasmic IL-8 in the decidual CD56bright NK cells was also detected by flow cytometry. RT-PCR methods confirmed IL-8 mRNA expression in this population, while no or very scarce expression of IL-1 alpha and IL-1 beta mRNA was observed. The present study is a first observation revealing that decidual CD16-CD56bright NK cells express IL-8 mRNA and produce IL-8.
在妊娠早期，使用荧光激活细胞分选仪从蜕膜单核细胞（MNC）中分离出蜕膜CD16-CD56bright自然杀伤（NK）细胞。与外周血MNC中非常小的比例（<1%）相比，CD16-CD56bright NK细胞群体在蜕膜MNC中占主要部分。这些蜕膜CD16-CD56bright NK细胞产生大量IL-8，即在无任何刺激物的24小时培养上清液中，IL-8平均水平为96.7±19.8 ng/ml，这与脂多糖刺激的外周血MNC产生的IL-8水平相当。大部分IL-8归因于蜕膜CD16-CD56bright NK细胞的产生。通过流式细胞术也检测到了蜕膜CD56bright NK细胞中的细胞质IL-8。RT-PCR方法证实了该群体中IL-8 mRNA的表达，而未观察到IL-1α和IL-1β mRNA的表达或表达非常稀少。本研究首次观察到蜕膜CD16-CD56bright NK细胞表达IL-8 mRNA并产生IL-8。
