Kennedy W R, Wendelschafer-Crabb G, Brelje T C
Department of Neurology, University of Minnesota, Minneapolis 55455.
J Neurosci. 1994 Nov;14(11 Pt 2):6825-33. doi: 10.1523/JNEUROSCI.14-11-06825.1994.
Secreting tubules, nerves fibers, and blood vessels in human sweat glands (SGs) were fluorescently stained by immunohistochemical and lectin methods for examination with a laser scanning confocal microscope (LSCM). Using these techniques, the three-dimensional distribution of up to three substances within a single specimen was investigated by collecting a series of optical sections for each of three fluorophores. Each SG received several nerve fibers. These branched into delicate bands of one or more axons that ran longitudinal to the sweat tubule then encircled the tubule. A heavy complement of capillaries was interwoven among the sweat tubules. Sweat ducts were accompanied from the SG toward the skin surface by one or two longitudinally oriented nerve fibers and capillaries. Immunoreactive staining of nerves was heaviest with protein gene product 9.5 antibody, but triple labeling showed that immunoreactivity to calcitonin gene-related peptide, vasoactive intestinal polypeptide, and synaptophysin was also present in the same axons. Substance P-immunoreactive axons were sparse in SGs but were present in other areas of the skin. The techniques used have considerable potential in examination of human skin biopsies for diagnosis of disorders affecting the somatic and autonomic nervous systems.
采用免疫组织化学和凝集素方法对人汗腺（SGs）中的分泌小管、神经纤维和血管进行荧光染色，以便用激光扫描共聚焦显微镜（LSCM）检查。利用这些技术，通过收集三种荧光团各自的一系列光学切片，研究单个标本中多达三种物质的三维分布。每个汗腺都接受数条神经纤维。这些神经纤维分支形成由一条或多条轴突组成的细带，这些轴突纵向延伸至汗腺小管，然后环绕小管。大量毛细血管交织在汗腺小管之间。从汗腺到皮肤表面的汗腺导管伴随着一两条纵向排列的神经纤维和毛细血管。用蛋白质基因产物9.5抗体对神经进行免疫反应性染色最强，但三重标记显示，同一轴突中也存在对降钙素基因相关肽、血管活性肠肽和突触素的免疫反应性。P物质免疫反应性轴突在汗腺中稀少，但存在于皮肤的其他区域。所使用的技术在检查人体皮肤活检组织以诊断影响躯体和自主神经系统的疾病方面具有相当大的潜力。
