Donadio Vincenzo, Nolano Maria, Provitera Vincenzo, Stancanelli Annamaria, Lullo Francesco, Liguori Rocco, Santoro Lucio
Department of Neurological Sciences, University of Bologna, Bologna, Naples, Italy.
Ann Neurol. 2006 Feb;59(2):376-81. doi: 10.1002/ana.20769.
The aim of this study was to characterize sympathetic adrenergic innervation of the skin in healthy subjects using dopamine beta hydroxylase (DbetaH) as a specific marker for noradrenergic fibers.
Sympathetic adrenergic innervation of human skin was studied in 10 healthy subjects by indirect immunofluorescence and confocal microscopy applied to punch skin biopsies. Noradrenergic fibers were identified both in glabrous and hairy skin using DbetaH antibody.
DbetaH immunoreactive fibers were mainly localized in arteriovenous anastomoses, arrector pilorum muscles, and arterioles, whereas few adrenergic fibers were found around sweat glands.
Our description of sympathetic adrenergic innervation of human skin aims to improve the diagnostic ability of skin biopsy to detect selective autonomic nervous system disorders.
本研究旨在以多巴胺β羟化酶（DβH）作为去甲肾上腺素能纤维的特异性标志物，对健康受试者皮肤的交感肾上腺素能神经支配进行特征描述。
通过间接免疫荧光和共聚焦显微镜对10名健康受试者的皮肤打孔活检标本进行研究，以观察人类皮肤的交感肾上腺素能神经支配。使用DβH抗体在无毛皮肤和有毛皮肤中识别去甲肾上腺素能纤维。
DβH免疫反应性纤维主要位于动静脉吻合处、立毛肌和小动脉，而在汗腺周围发现的肾上腺素能纤维较少。
我们对人类皮肤交感肾上腺素能神经支配的描述旨在提高皮肤活检检测选择性自主神经系统疾病的诊断能力。
