Bai M C, Jiwa N M, Horstman A, Vos W, Kluin P H, Van der Valk P, Mullink H, Walboomers J M, Meijer C J
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
J Pathol. 1994 Sep;174(1):49-55. doi: 10.1002/path.1711740108.
Epstein-Barr virus (EBV) has been demonstrated in the Reed-Sternberg cells and their mononuclear variants (Hodgkin cells; H-RS cells) in a substantial number of Hodgkin's disease (HD) cases. Moreover, EBV can modulate both in vivo and in vitro the expression of several cellular genes, including lymphoid differentiation markers. Therefore we investigated, in 64 cases of HD, the relationship between the presence of EBV and the expression of lymphoid (CD45RB), T- (CD3, CD45RO), B- (CD20, MB2 antigen, CDw75), and myeloid-cell lineage markers (CD15), and of activation markers (CD30, EMA, and the 115D8 antigen) on the H-RS cells. EBV-positive cases, as demonstrated by the presence of EBER-1 and -2 RNA and LMP-1 protein expression, showed a significant reduction in the expression on H-RS cells of T-cell lineage (CD3, P < 0.02), B-cell lineage (CD20; P < 0.005), and activation markers (EMA; P < 0.002 and the 115D8 antigen; P < 0.001) as compared with EBV-negative cases. No differences were found in the expression of CD15, CD30, CD45RO, CD45RB, CDw75, or the MB2 antigen on H-RS cells in EBV-positive and EBV-negative HD cases. Interestingly, in 11 cases of EBV-negative HD, B- as well as T-cell lineage markers could be found on some H-RS cells. These data suggest that EBV in H-RS cells is able to down-regulate the expression of T- (CD3) and B- (CD20) cell lineage markers and lymphoid activation markers (EMA and the 115D8 antigen).(ABSTRACT TRUNCATED AT 250 WORDS)
在大量霍奇金淋巴瘤（HD）病例的里德-斯特恩伯格细胞及其单核变体（霍奇金细胞；H-RS细胞）中已证实存在爱泼斯坦-巴尔病毒（EBV）。此外，EBV在体内和体外均可调节多种细胞基因的表达，包括淋巴细胞分化标志物。因此，我们在64例HD病例中研究了EBV的存在与H-RS细胞上淋巴细胞（CD45RB）、T细胞（CD3、CD45RO）、B细胞（CD20、MB2抗原、CDw75）和髓细胞系标志物（CD15）以及活化标志物（CD30、EMA和115D8抗原）表达之间的关系。通过EBER-1和-2 RNA以及LMP-1蛋白表达证明为EBV阳性的病例，与EBV阴性病例相比，H-RS细胞上T细胞系（CD3，P<0.02）、B细胞系（CD20；P<0.005）和活化标志物（EMA；P<0.002和115D8抗原；P<0.001）的表达显著降低。在EBV阳性和EBV阴性HD病例的H-RS细胞上，CD15、CD30、CD45RO、CD45RB、CDw75或MB2抗原的表达未发现差异。有趣的是，在11例EBV阴性HD病例中，在一些H-RS细胞上可发现B细胞和T细胞系标志物。这些数据表明，H-RS细胞中的EBV能够下调T细胞（CD3）和B细胞（CD20）系标志物以及淋巴细胞活化标志物（EMA和115D8抗原）的表达。（摘要截短至250字）
