Jones J L, Berth-Jones J, Fletcher A, Hutchinson P E
Department of Histopathology, Leicester Royal Infirmary, U.K.
J Pathol. 1994 Oct;174(2):77-82. doi: 10.1002/path.1711740203.
Epidermal dendritic and T-cell counts have been performed in lesional and non-lesional skin from 35 psoriatic patients. The aims were to investigate absolute changes and interrelationships between these cellular elements in psoriasis and to explain apparent discrepancies between these results and reports in the literature. In non-lesional skin, the most frequently expressed dendritic cell marker was CD1a. HLA-DR+ and alpha-mannosidase+ dendritic cells were approximately 50 per cent and S100+ cells were 25 per cent as frequent. T-lymphocytes were rare, CD4+ cells predominating. In lesional psoriatic epidermis, there was a definite increase (approximately two-fold) in the absolute number of CD1a+ dendritic cells. This differs from the conclusions from the majority of previous studies. However, when cell counts were expressed per unit area of vertical section, there was a decrease in CD1a+ cells in lesional skin, which is an explanation for this discrepancy. There was a greater increase in absolute HLA-DR+ cell counts, so that the numbers of cells expressing CD1a and HLA-DR were similar in lesional skin. S100 expression increased proportionately with CD1a+, but there was no absolute increase in alpha-mannosidase+ cells, which might represent a separate sub-population of dendritic cells. The greatest cellular increase was in T-lymphocytes, particularly CD8+. In lesional skin, direct correlations have been demonstrated between epidermal thickness, HLA-DR+ dendritic cells and T-lymphocytes, particularly CD8+ cells. We would suggest that the present method of quantification is of value for the analysis of absolute changes in epidermal infiltrates, particularly psoriasis, and could be applied to other epidermal pathologies.
对35例银屑病患者的皮损和非皮损皮肤进行了表皮树突状细胞和T细胞计数。目的是研究银屑病中这些细胞成分之间的绝对变化和相互关系，并解释这些结果与文献报道之间明显的差异。在非皮损皮肤中，最常表达的树突状细胞标志物是CD1a。HLA-DR+和α-甘露糖苷酶+树突状细胞的频率约为50%，S100+细胞的频率为25%。T淋巴细胞很少见，以CD4+细胞为主。在银屑病皮损表皮中，CD1a+树突状细胞的绝对数量有明显增加（约两倍）。这与大多数先前研究的结论不同。然而，当按垂直切片的单位面积进行细胞计数时，皮损皮肤中CD1a+细胞数量减少，这解释了这种差异。HLA-DR+细胞的绝对计数增加得更多，因此在皮损皮肤中表达CD1a和HLA-DR的细胞数量相似。S100表达与CD1a+成比例增加，但α-甘露糖苷酶+细胞没有绝对增加，这可能代表树突状细胞的一个单独亚群。细胞增加最多的是T淋巴细胞，尤其是CD8+。在皮损皮肤中，已证实表皮厚度、HLA-DR+树突状细胞和T淋巴细胞，尤其是CD8+细胞之间存在直接相关性。我们认为，目前的定量方法对于分析表皮浸润，特别是银屑病中的绝对变化具有价值，并且可以应用于其他表皮病变。
