Gobbetti A, Zerani M, Di Fiore M M, Botte V
Department of Molecular, Cellular and Animal Biology, Camerino MC, Italy.
J Reprod Fertil. 1994 Aug;101(3):523-9. doi: 10.1530/jrf.0.1010523.
The release of PGF2 alpha and PGE2, progesterone, androgens and oestradiol in vitro, and the aromatase activity in the brain of the male lizard Podarcis sicula sicula during three different phases of the reproductive period were evaluated. In addition, the effects of salmon GnRH, substance P, salmon GnRH antagonist, substance P antagonist, PGF2 alpha, PGE2 and acetylsalicylic acid on the release of prostaglandins and sex steroids and on aromatase activity in the brain were evaluated during the same three phases. PGF2 alpha, oestradiol and aromatase activity were higher during the refractory phase, androgens during the fighting phase, and progesterone during the mating phase, while PGE2 was lower during the refractory phase. Treatment with salmon GnRH increased PGF2 alpha, oestradiol and aromatase activity, but decreased the amount of androgens released. Substance P decreased PGF2 alpha, oestradiol and aromatase activity, but increased the amount of androgens released. PGF2 alpha increased oestradiol and aromatase activity, but decreased the amount of androgens released. Acetylsalicylic acid decreased PGF2 alpha, oestradiol and aromatase activity, but increased the amount of androgens released. These data suggest that salmon GnRH and substance P have different roles in reproductive processes, with opposite mechanisms, in the central nervous system of this male lizard: salmon GnRH seems to be involved in regulating the refractory phase, while substance P plays a role in regulating the fighting phase.
评估了雄性意大利壁蜥（Podarcis sicula sicula）在生殖期三个不同阶段体外前列腺素F2α（PGF2α）、前列腺素E2（PGE2）、孕酮、雄激素和雌二醇的释放情况，以及大脑中的芳香化酶活性。此外，还评估了在相同的三个阶段中，鲑鱼促性腺激素释放激素（GnRH）、P物质、鲑鱼GnRH拮抗剂、P物质拮抗剂、PGF2α、PGE2和乙酰水杨酸对前列腺素和性类固醇释放以及大脑中芳香化酶活性的影响。PGF2α、雌二醇和芳香化酶活性在不应期较高，雄激素在争斗期较高，孕酮在交配期较高，而PGE2在不应期较低。用鲑鱼GnRH处理可增加PGF2α、雌二醇和芳香化酶活性，但会减少雄激素的释放量。P物质可降低PGF2α、雌二醇和芳香化酶活性，但会增加雄激素的释放量。PGF2α可增加雌二醇和芳香化酶活性，但会减少雄激素的释放量。乙酰水杨酸可降低PGF2α、雌二醇和芳香化酶活性，但会增加雄激素的释放量。这些数据表明，在这种雄性蜥蜴的中枢神经系统中，鲑鱼GnRH和P物质在生殖过程中具有不同的作用，其机制相反：鲑鱼GnRH似乎参与调节不应期，而P物质在调节争斗期发挥作用。
