Gobbetti A, Zerani M, Bellini-Cardellini L, Bolelli G F
Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
Acta Physiol Scand. 1995 Mar;153(3):301-8. doi: 10.1111/j.1748-1716.1995.tb09866.x.
The in vitro effects of prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2) on corticosterone release by ovarian follicles, corpora lutea (CL), and interrenals were studied in the female lizard, Podarcis sicula sicula, during reproduction. Follicles and CL studied in the female lizard, Podarcis sicula sicula, during reproduction. Follicles and CL were divided according to their different developmental stages; follicles: previtellogenic, early-vitellogenic, mid-vitellogenic and fully-grown; CL: CL1 (unshelled eggs in the oviducts), CL2 (shelled eggs in the oviducts), CL3 (eggs laid 6 h previously) and CL4 (eggs laid 48 h previously). Interrenals were divided according to the reproductive stages: pre-vitellogenesis, vitellogenesis, ovulation, post-ovulation, and post-deposition. PGF2 alpha release was highest in fully-grown follicles and PGE2 in early-vitellogenic follicles, corticosterone was highest in pre-vitellogenic and lowest in early-vitellogenic follicles. PGE2 decreased corticosterone in pre-vitellogenic, mid-vitellogenic and fully-grown follicles. PGF2 alpha release was highest in CL4, and PGE2 in CL1 and CL2, corticosterone was highest in CL4. PGF2 alpha increased corticosterone in CL1, CL2 and CL3. In interrenals, PGF2 alpha release was highest and PGE2 lowest during ovulation, corticosterone was highest during ovulation. PGF2 alpha increased and PGE2 decreased interrenal corticosterone during vitellogenesis, ovulation, and post-ovulation. In the plasma, PGF2 alpha levels were highest and PGE2 lowest during ovulation, corticosterone was highest during ovulation. These results suggest that corticosterone, modulated by PGF2 alpha and PGE2, is implied in the reproductive processes with different roles. In fact this steroid could favour ovulatory and luteolytic processes. In addition the hypothesis of an anti-vitellogenic role of corticosterone is discussed.
在繁殖期间，对雌性意大利壁蜥（Podarcis sicula sicula）研究了前列腺素F2α（PGF2α）和前列腺素E2（PGE2）对卵巢卵泡、黄体（CL）和肾上腺皮质释放皮质酮的体外影响。在繁殖期间对雌性意大利壁蜥的卵泡和黄体进行了研究。卵泡和黄体根据其不同发育阶段进行划分；卵泡：卵黄生成前期、卵黄生成早期、卵黄生成中期和完全成熟卵泡；黄体：CL1（输卵管内未产卵壳的卵）、CL2（输卵管内已产卵壳的卵）、CL3（6小时前产下的卵）和CL4（48小时前产下的卵）。肾上腺根据生殖阶段进行划分：卵黄生成前期、卵黄生成期、排卵期、排卵后期和产卵后期。完全成熟卵泡中PGF2α释放量最高，卵黄生成早期卵泡中PGE2释放量最高，卵黄生成前期卵泡中皮质酮含量最高，卵黄生成早期卵泡中皮质酮含量最低。PGE2降低了卵黄生成前期、卵黄生成中期和完全成熟卵泡中的皮质酮含量。CL4中PGF2α释放量最高，CL1和CL2中PGE2释放量最高，CL4中皮质酮含量最高。PGF2α增加了CL1、CL2和CL3中的皮质酮含量。在肾上腺中，排卵期间PGF2α释放量最高，PGE2释放量最低，排卵期间皮质酮含量最高。在卵黄生成期、排卵期和排卵后期，PGF2α增加而PGE2降低肾上腺皮质酮含量。在血浆中，排卵期间PGF2α水平最高，PGE2水平最低，排卵期间皮质酮含量最高。这些结果表明，由PGF2α和PGE2调节的皮质酮在生殖过程中具有不同作用。事实上，这种类固醇可能有利于排卵和黄体溶解过程。此外，还讨论了皮质酮具有抗卵黄生成作用的假说。
