Gobbetti A, Zerani M, Di Fiore M M
Department of Molecular, Cellular, and Animal Biology, University of Camerino, Italy.
Gen Comp Endocrinol. 1994 Feb;93(2):153-62. doi: 10.1006/gcen.1994.1018.
The in vitro effects of salmon gonadotropin-releasing hormone (sGnRH), substance P (SP), and their antagonists on prostaglandin F2 alpha (PGF2 alpha), prostaglandin E2 (PGE2), progesterone, androgens, and estradiol-17 beta release by follicles and corpus luteum (CL) of the oviparous lizard, Podarcis sicula sicula, were studied. Follicles and CL were divided according to the different developmental stages; follicles: pre-vitellogenic, early-vitellogenic, mid-vitellogenic, and fully grown; CL: CL1 (unshelled eggs in the oviducts), CL2 (shelled eggs in the oviducts), CL3 (eggs laid 6 hr previously), and CL4 (eggs laid 48 hr previously). SGnRH increased PGF2 alpha and progesterone release by mid-vitellogenic and fully grown follicles; SP increased PGE2 and estradiol-17 beta release by pre-vitellogenic, mid-vitellogenic, and fully grown follicles. SGnRH and SP decreased PGE2 and progesterone and increased PGF2 alpha by CL1 and CL2. The antagonists of these two neuropeptides induced the opposite effects of those of sGnRH and SP. The present data indicate that sGnRH and SP play different roles in the regulation of prostaglandins and sex steroid production by ovarian follicles and CL of P. s. sicula.
研究了鲑鱼促性腺激素释放激素（sGnRH）、P物质（SP）及其拮抗剂对卵生蜥蜴西西里岛壁蜥卵泡和黄体（CL）释放前列腺素F2α（PGF2α）、前列腺素E2（PGE2）、孕酮、雄激素和雌二醇-17β的体外作用。卵泡和CL根据不同发育阶段进行划分；卵泡：卵黄生成前、卵黄生成早期、卵黄生成中期和完全成熟；CL：CL1（输卵管中未产卵壳的卵）、CL2（输卵管中已产卵壳的卵）、CL3（6小时前产下的卵）和CL4（48小时前产下的卵）。sGnRH增加了卵黄生成中期和完全成熟卵泡中PGF2α和孕酮的释放；SP增加了卵黄生成前、卵黄生成中期和完全成熟卵泡中PGE2和雌二醇-17β的释放。sGnRH和SP降低了CL1和CL2中PGE2和孕酮的含量，并增加了PGF2α的含量。这两种神经肽的拮抗剂诱导了与sGnRH和SP相反的作用。目前的数据表明，sGnRH和SP在调节西西里岛壁蜥卵巢卵泡和CL的前列腺素和性类固醇产生中发挥不同作用。
