Frank J M, Kaneko S, Joels C, Tobin G R, Banis J C, Barker J H
Department of Trauma Surgery, University of Saarland, Germany.
Microsurgery. 1994;15(6):399-404. doi: 10.1002/micr.1920150607.
Angiogenesis, the formation of new blood vessels, is essential to a variety of normal and pathologic processes such as wound healing and tumor growth. In microsurgery the development of new vessels between the transferred tissue and the recipient bed is critical to the final outcome of the reconstruction. Several experimental models have been previously developed to study angiogenesis and the effect that new substances have on regulating this process, but they lack the ability to make quantitative measurements. Therefore, we have developed an animal model using the homozygous (hr/hr) hairless mouse ear; by using intravital microscopy and computer-assisted analysis, angiogenesis can be quantitatively measured. Using this model we showed that basic fibroblast growth factor and transforming growth factor beta significantly increased total vessel length by 32% and 63%, respectively, during 20 days following subcutaneous injection. In this paper the importance of angiogenesis research to reconstructive microsurgery is presented and discussed.
血管生成，即新血管的形成，对于诸如伤口愈合和肿瘤生长等各种正常和病理过程至关重要。在显微外科手术中，移植组织与受区之间新血管的形成对重建的最终结果至关重要。先前已经开发了几种实验模型来研究血管生成以及新物质对调节这一过程的影响，但它们缺乏进行定量测量的能力。因此，我们开发了一种使用纯合（hr/hr）无毛小鼠耳朵的动物模型；通过活体显微镜检查和计算机辅助分析，可以对血管生成进行定量测量。使用该模型我们发现，在皮下注射后的20天内，碱性成纤维细胞生长因子和转化生长因子β分别使血管总长度显著增加了32%和63%。本文介绍并讨论了血管生成研究对重建显微外科手术的重要性。
