Bondár I, Uhl E, Barker J H, Galla T J, Hammersen F, Messmer K
Department of Experimental Surgery, University of Heidelberg, Federal Republic of Germany.
Res Exp Med (Berl). 1991;191(6):379-88. doi: 10.1007/BF02576693.
Intact blood supply by microcirculation to a wounded site is an indispensable prerequisite for normal tissue regeneration. However, microvascular changes taking place in the healing process of skin wounds are not understood due to the fact that only few models allow chronic in vivo studies on skin microcirculation. Therefore, we have modified the hairless mouse ear model with the purpose of a quantitative in vivo study of microhemodynamic changes throughout the healing process. Following the creation of a standardized skin wound on the ear of the homozygous hairless mouse (hr/hr), microvessel diameters, red blood cell velocities, wet weight, and leucocyte content of the ear tissue were determined. Surface area of the wound was assessed until complete closure was achieved. By repeated measurements at identical sites over the entire healing period, a distinct pattern of microvascular changes could be observed: microvessel diameters increased to a maximum a few days after wound creation, whereas red blood cell velocities reached their highest values at a later point in time and were still elevated after complete reepithelization of the wounds. Edema and leucocyte content of the ear tissue was most prominent in the early healing phase and gradually decreased to normal values thereafter. These results demonstrate changes of the microvasculature of the hairless mouse ear to injury, which are in accordance to other more indirect studies on this topic. Therefore, we conclude that the model presented is suitable for prolonged quantitative analysis of microcirculation during normal wound healing and may be used to assess microvascular changes taking place during wound healing in pathologically altered tissue.
通过微循环向受伤部位提供完整的血液供应是正常组织再生必不可少的前提条件。然而，由于只有少数模型允许对皮肤微循环进行慢性体内研究，因此皮肤伤口愈合过程中发生的微血管变化尚不清楚。因此，我们对无毛小鼠耳模型进行了改良，目的是对整个愈合过程中的微观血流动力学变化进行定量体内研究。在纯合无毛小鼠（hr/hr）的耳朵上制造标准化皮肤伤口后，测定耳组织的微血管直径、红细胞速度、湿重和白细胞含量。评估伤口表面积直至完全闭合。通过在整个愈合期内在相同部位重复测量，可以观察到微血管变化的明显模式：微血管直径在伤口形成后几天增加到最大值，而红细胞速度在稍后的时间点达到最高值，并且在伤口完全重新上皮化后仍保持升高。耳组织的水肿和白细胞含量在愈合早期最为明显，此后逐渐降至正常值。这些结果证明了无毛小鼠耳微血管对损伤的变化，这与关于该主题的其他更间接的研究一致。因此，我们得出结论，所提出的模型适用于正常伤口愈合期间微循环的长期定量分析，并且可用于评估病理改变组织中伤口愈合期间发生的微血管变化。
