Böttjer J, Amon U, Wolff H H
Department of Dermatology, Medical University of Lübeck, Germany.
Agents Actions. 1994 Jun;41 Spec No:C28-9. doi: 10.1007/BF02007752.
The present study was performed to investigate the histamine-releasing activity of non-immunological stimuli on cultured mast cell lines in comparison to isolated skin mast cells and basophils as human therapeutic target cells. The ionophore A23187 induced a dose dependent histamine release from all cell populations (enzymatically isolated human skin mast cells, human peripheral basophils and rat basophilic leukemia cells, RBL-1 and RBL-2H3). The lectin concanavalin A and the tripeptide formyl-methionyl-leucyl-phenylalanine activated only basophils, while the neural mediator substance P and compound 48/80 were active only in experiments with skin mast cells. Activators of protein kinase C (different phorbol esters and the non-phorbol mezerein) induced direct histamine release only from basophils. The data provide further evidence for heterogeneity of mast cells and indicate different signal transduction mechanisms following non-immunological activation.
本研究旨在比较非免疫刺激对培养的肥大细胞系、分离的皮肤肥大细胞和嗜碱性粒细胞（作为人类治疗靶细胞）的组胺释放活性。离子载体A23187诱导所有细胞群体（酶法分离的人皮肤肥大细胞、人外周嗜碱性粒细胞以及大鼠嗜碱性白血病细胞RBL-1和RBL-2H3）呈剂量依赖性组胺释放。凝集素伴刀豆球蛋白A和三肽甲酰甲硫氨酰亮氨酰苯丙氨酸仅激活嗜碱性粒细胞，而神经介质P物质和化合物48/80仅在皮肤肥大细胞实验中具有活性。蛋白激酶C激活剂（不同佛波酯和非佛波酯芫花酯）仅诱导嗜碱性粒细胞直接释放组胺。这些数据为肥大细胞的异质性提供了进一步证据，并表明非免疫激活后存在不同的信号转导机制。
