Assem E S, Ghanem N S, Abdullah N A
Agents Actions. 1987 Feb;20(1-2):1-9. doi: 10.1007/BF01965619.
A comparison was made between collagenase-dispersed guinea-pig atrial and ventricular tissues. Heparin containing cells were stained with alcian blue at pH 2.2, and counted by an automated technique (Technicon H6000). The cells were challenged with the specific antigen (ovalbumin), with antisera to guinea-pig IgG (non-subclass specific), IgG1 and IgG2, and the calcium ionophore A23187. Histamine release was measured by an automated spectrofluorometric technique, and leukotriene C4 was measured by radioimmunoassay. All of the following parameters were higher in the atrial than in ventricular cells (mean ratio and SEM of atrial: ventricular mast cell parameters in parenthesis): Histamine content/g wet tissues (3.32 +/- 0.71:1) (p less than 0.05), Absolute mast cell number as a proportion of total cell count (3.75 +/- 1.64:1), Histamine release induced by antigen (significant in one out of four experiments), anti-IgG (significant in three out of four experiments), anti-IgG1 (significant in two out of four experiments), and anti-IgG2 (higher but not statistically significant). Ionophore A23187 gave an inconsistent histamine release pattern: significantly higher release from atria in five treatments (different concentrations in different experiments), and higher ventricular release in three. Significantly more leukotriene C4 was released by antigen and the ionophore A23187 (mean of 3-5 treatments), but not with anti-IgG.
对胶原酶分散的豚鼠心房和心室组织进行了比较。含肝素的细胞在pH 2.2条件下用阿尔辛蓝染色，并通过自动技术（Technicon H6000）计数。用特异性抗原（卵清蛋白）、抗豚鼠IgG（非亚类特异性）、IgG1和IgG2的抗血清以及钙离子载体A23187刺激这些细胞。组胺释放通过自动荧光分光光度技术测量，白三烯C4通过放射免疫测定法测量。以下所有参数在心房细胞中均高于心室细胞（括号内为心房：心室肥大细胞参数的平均比值和标准误）：每克湿组织中的组胺含量（3.32±0.71:1）（p＜0.05），肥大细胞绝对数占总细胞数的比例（3.75±1.64:1），抗原诱导的组胺释放（4次实验中有1次显著）、抗IgG（4次实验中有3次显著）、抗IgG1（4次实验中有2次显著）以及抗IgG2（较高但无统计学意义）。离子载体A23187产生的组胺释放模式不一致：在5种处理中（不同实验中浓度不同）心房释放显著更高，在3种处理中心室释放更高。抗原和离子载体A23187释放的白三烯C4显著更多（3 - 5种处理的平均值），但抗IgG处理时并非如此。
