Likovský Z, Peterka M, Peterková R
Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague.
Anat Embryol (Berl). 1994 Jun;189(6):539-44. doi: 10.1007/BF00186827.
The primitive erythroid line cells of chick embryos were studied during embryonic days 2-14 by means of a cytochemical method to investigate the appearance and frequency of the main nucleolar types. The populations of erythroblasts and erythrocytes were classified according to the presence of functionally dominant nucleoli in their nuclei. In the course of primitive erythroid cell differentiation and maturation, compact nucleoli and nucleoli with nucleolonemas (both supposed to be RNA biosynthetically active) were gradually replaced by ring-shaped nucleoli and finally by micronucleoli reflecting the reversible and irreversible inhibition of RNA synthesis, respectively. The occurrence of the main nucleolar types and their values in primitive erythroid cells of the developing chick depend not only on the maturation stage of the blood cells, but also on the developmental stage of the chick embryo. In comparison with the definitive erythroid line of the post-hatching chick and hen, the cells of the chick embryonic primitive erythroid line possess relatively high values of "active" nucleolar types. These are still present in advanced maturation stages, and occur also as definitive erythroid lines of lower vertebrates.
在胚胎发育的第2至14天，利用细胞化学方法对鸡胚的原始红细胞系细胞进行了研究，以探究主要核仁类型的出现情况和频率。根据成红细胞和成红细胞细胞核中功能性显性核仁的存在情况，对其群体进行了分类。在原始红细胞的分化和成熟过程中，致密核仁和带有核仁丝的核仁（两者均被认为具有活跃的RNA生物合成功能）逐渐被环形核仁取代，最终被微核仁取代，这分别反映了RNA合成的可逆性抑制和不可逆性抑制。发育中鸡胚原始红细胞中主要核仁类型的出现及其数值不仅取决于血细胞的成熟阶段，还取决于鸡胚的发育阶段。与孵化后雏鸡和母鸡的定型红细胞系相比，鸡胚原始红细胞系的细胞具有相对较高的“活跃”核仁类型数值。这些数值在晚期成熟阶段仍然存在，并且在低等脊椎动物的定型红细胞系中也会出现。
