Powell P P, Finklestein S P, Dionne C A, Jaye M, Klagsbrun M
Department of Surgery, Children's Hospital, Harvard Medical School, Boston, MA 02115.
Brain Res Mol Brain Res. 1991 Aug;11(1):71-7. doi: 10.1016/0169-328x(91)90023-q.
The expression of basic fibroblast growth factor (bFGF) mRNA and bFGF receptor mRNA was investigated in developing rat brain. In embryonic rat brain days 13-21 (E13-E21), an abundant 1.8 kb bFGF mRNA was detected. Expression of 1.8 kb bFGF mRNA was the highest at E17 to E19 and was relatively undetectable 20 days after birth. However, very little mitogenic activity was associated with prenatal brain. On the other hand, multiple bFGF mRNA species of 6.0, 3.7, 2.5, 1.8, 1.6, 1.4 and 1.0 kb were detected in total adult rat brain and a significant amount of mitogenic activity was present. Differential and spatial bFGF mRNA expression was found in different parts of developing rat brain. Embryonic hypothalamus was found to contain the 1.8 kb bFGF mRNA while the 6.0 kb bFGF mRNA transcript was predominant in adult hypothalamus. Adult pituitary and cortex transcribed the lower molecular weight mRNAs but not the 6.0 kb mRNA. Expression of high-affinity bFGF receptor (flg) mRNA was found to be temporally regulated. flg 4.3 kb mRNA expression was high in embryonic rat brain (E13-E19). There appears to be coordinate expression between the 1.8 kb bFGF mRNA and flg. These results suggest that the expression of basic FGF mRNA is complex since it is both temporally and differentially regulated with different species being expressed at different times in development.
研究了碱性成纤维细胞生长因子（bFGF）mRNA和bFGF受体mRNA在发育中的大鼠脑中的表达情况。在胚胎大鼠脑发育的第13至21天（E13 - E21），检测到大量1.8 kb的bFGF mRNA。1.8 kb bFGF mRNA的表达在E17至E19时最高，出生后20天相对难以检测到。然而，产前脑的促有丝分裂活性非常低。另一方面，在成年大鼠全脑中检测到了6.0、3.7、2.5、1.8、1.6、1.4和1.0 kb等多种bFGF mRNA种类，并且存在大量促有丝分裂活性。在发育中的大鼠脑的不同部位发现了bFGF mRNA的差异表达和空间表达。胚胎下丘脑含有1.8 kb的bFGF mRNA，而6.0 kb的bFGF mRNA转录本在成年下丘脑中占主导。成年垂体和皮质转录较低分子量的mRNA，但不转录6.0 kb的mRNA。发现高亲和力bFGF受体（flg）mRNA的表达受到时间调控。flg 4.3 kb mRNA在胚胎大鼠脑（E13 - E19）中的表达较高。1.8 kb bFGF mRNA和flg之间似乎存在协同表达。这些结果表明，碱性FGF mRNA的表达很复杂，因为它在时间上和差异上都受到调控，不同种类在发育的不同时间表达。
