Akridge R E, Oyafuso L K, Reed S G
Infectious Disease Research Institute, Seattle, WA 98104.
J Immunol. 1994 Dec 15;153(12):5782-9.
IL-10 has been shown to be capable of down-regulating several aspects of macrophage function. This study was undertaken to define the association between IL-10 and HIV-1 infection in human macrophages. Infection of macrophages with a monocytotropic strain of the human immunodeficiency virus, HIV-BaL, resulted in expression of IL-10 mRNA within 3 to 12 h after infection, as determined by the reverse transcriptase PCR. Biologically active IL-10 was detected in supernatants from HIV-1-infected macrophages as early as 12 h post-infection. The addition of human rIL-10 to HIV-1-infected macrophage cultures resulted in a significant decrease in the viral replication. In addition, exogenous IL-10 blocked the ability of TNF-alpha to elevate viral replication. To determine whether IL-10 was associated with in vivo infection, lymph nodes from AIDS patients were examined for the presence of IL-10 mRNA by using PCR. IL-10 mRNA was evident in all lymph node tissue examined, but was absent in normal lymph node biopsies. These in vitro and in vivo findings demonstrate a strong and heterogeneous association between HIV-1 infection and IL-10.
白细胞介素-10已被证明能够下调巨噬细胞功能的多个方面。本研究旨在确定白细胞介素-10与人类巨噬细胞中HIV-1感染之间的关联。用人免疫缺陷病毒的嗜单核细胞株HIV-BaL感染巨噬细胞，通过逆转录酶PCR测定，感染后3至12小时内白细胞介素-10信使核糖核酸开始表达。早在感染后12小时，在HIV-1感染的巨噬细胞的上清液中就检测到了具有生物活性的白细胞介素-10。将人重组白细胞介素-10添加到HIV-1感染的巨噬细胞培养物中，病毒复制显著减少。此外，外源性白细胞介素-10阻断了肿瘤坏死因子-α提高病毒复制的能力。为了确定白细胞介素-10是否与体内感染有关，通过PCR检测了艾滋病患者淋巴结中白细胞介素-10信使核糖核酸的存在情况。在所检查的所有淋巴结组织中都能明显检测到白细胞介素-10信使核糖核酸，但在正常淋巴结活检组织中未检测到。这些体外和体内研究结果表明HIV-1感染与白细胞介素-10之间存在强烈且异质性的关联。
