Müller L, Kasper P, Müller-Tegethoff K, Petr T
Federal Institute for Drugs and Medical Devices, Berlin, Germany.
Mutat Res. 1994 Dec;325(4):129-36. doi: 10.1016/0165-7992(94)90075-2.
Estragole, trans-anethole and basil oil were tested for their ability to induce DNA repair in rat hepatocytes in vitro and in rat liver in an ex vivo test. There was a marked induction of UDS by estragole and basil oil in vitro (LOEC about 10(-5) mol/l). The basil oil we used contained about 88.2% estragole. It is evident from our results that the induction of UDS with basil oil could be directly related to its main constituent estragole. trans-Anethole was only slightly effective in the in vitro UDS test. The ex vivo UDS test led to clearly elevated DNA repair for estragole and basil oil in rats treated orally with doses up to 2 g/kg body weight. Estragole was not positive in a chromosomal aberration test with V79 cells either via direct treatment, with rat liver S9 mix or with rat hepatocytes as source of metabolism.
在体外实验中，对大鼠肝细胞以及在离体实验中对大鼠肝脏，测试了草蒿脑、反式茴香脑和罗勒油诱导DNA修复的能力。草蒿脑和罗勒油在体外显著诱导了非程序DNA合成（LOEC约为10⁻⁵ mol/l）。我们使用的罗勒油含有约88.2%的草蒿脑。从我们的结果可以明显看出，罗勒油诱导非程序DNA合成可能与其主要成分草蒿脑直接相关。反式茴香脑在体外非程序DNA合成试验中仅有轻微效果。在离体非程序DNA合成试验中，给大鼠口服高达2 g/kg体重的剂量后，草蒿脑和罗勒油可使DNA修复明显升高。在V79细胞的染色体畸变试验中，无论是直接处理、使用大鼠肝脏S9混合物还是以大鼠肝细胞作为代谢来源，草蒿脑均未呈阳性。
