Koriem Khaled M M, Arbid Mahmoud S, El-Gendy Nadia F
a Department of Medical Physiology , Medical Research Division , National Research Centre, Dokki , Cairo , Egypt.
b Department of Pharmacology , Medical Research Division , National Research Centre, Dokki , Cairo , Egypt.
J Diet Suppl. 2016;13(5):505-21. doi: 10.3109/19390211.2015.1119775. Epub 2016 Jan 8.
The metabolic disease favism is an acute hemolytic anemia. Anise oil was obtained from Pimpinella anisum L. seeds (family Apiaceae). The objective of this study was to establish the protective effect of anise oil in favism disorders. Forty-eight male albino rats were divided into six groups: group 1 orally administrated 1 mL distilled water, group 2 orally received 300 mg/kg anise oil, and group 3 orally administrated 100 mg/kg anethole over a seven-day period, group 4 favism-induced rats, group 5 orally administrated 300 mg/kg anise oil and group 6 orally administrated 100 mg/kg anethole once a day over a seven-day period prior to favism induction. The result obtained revealed that oral administration of either anise oil or anethole into normal rats over a seven-day period did not induce any change. Following favism induction, hemoglobin, hematocrit, red and white blood cell counts, serum glucose, blood glutathione, glucose-6-phosphate dehydrogenase, total protein, globulin, alanine and aspartate aminotransferases levels were significantly decreased, while serum alkaline phosphatase and bilirubin showed significant increase. Pretreatment with either anise oil or anethole into favism-induced rats prevented these changes. Favism also induced deoxyribonucleic acid (DNA) damage and prior treatment of anise oil maintained liver DNA content. These results were supported by histopathological evaluation. In conclusion, anise oil pretreatment into favism-induced rats decreased the favism disorders, and this effect was related to the anethole ingredient of the oil.
代谢性疾病蚕豆病是一种急性溶血性贫血。茴芹油是从茴芹（伞形科）种子中提取的。本研究的目的是确定茴芹油对蚕豆病的保护作用。48只雄性白化大鼠被分为6组：第1组口服1 mL蒸馏水，第2组口服300 mg/kg茴芹油，第3组在7天内口服100 mg/kg茴香脑，第4组为诱导蚕豆病的大鼠，第5组在诱导蚕豆病前7天每天口服300 mg/kg茴芹油，第6组在诱导蚕豆病前7天每天口服100 mg/kg茴香脑。结果显示，在7天内给正常大鼠口服茴芹油或茴香脑均未引起任何变化。诱导蚕豆病后，血红蛋白、血细胞比容、红细胞和白细胞计数、血清葡萄糖、血液谷胱甘肽、葡萄糖-6-磷酸脱氢酶、总蛋白、球蛋白、丙氨酸和天冬氨酸转氨酶水平显著降低，而血清碱性磷酸酶和胆红素显著升高。用茴芹油或茴香脑对诱导蚕豆病的大鼠进行预处理可防止这些变化。蚕豆病还会导致脱氧核糖核酸（DNA）损伤，茴芹油预处理可维持肝脏DNA含量。这些结果得到了组织病理学评估的支持。总之，对诱导蚕豆病的大鼠进行茴芹油预处理可减轻蚕豆病症状，且这种作用与茴芹油中的茴香脑成分有关。
