核糖核酸酶H在大肠杆菌的逆转录子Ec73和逆转录子Ec107多拷贝单链DNA合成中的作用。
The role of ribonuclease H in multicopy single-stranded DNA synthesis in retron-Ec73 and retron-Ec107 of Escherichia coli.
作者信息
Shimamoto T, Shimada M, Inouye M, Inouye S
机构信息
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
出版信息
J Bacteriol. 1995 Jan;177(1):264-7. doi: 10.1128/jb.177.1.264-267.1995.
Abstract
Bacterial reverse transcriptase is responsible for the synthesis of multicopy single-stranded DNA (msDNA). Reverse transcriptases from retron-Ec73 and retron-Ec107 do not contain an RNase H domain. Cellular RNase H is therefore considered to be required to make the mature form of msDNA. We found that RNase HI, but not RNase HII, is required for the production of the mature form of both msDNAs.
摘要
细菌逆转录酶负责多拷贝单链DNA（msDNA）的合成。来自逆转录子Ec73和逆转录子Ec107的逆转录酶不包含核糖核酸酶H结构域。因此，细胞核糖核酸酶H被认为是产生成熟形式的msDNA所必需的。我们发现，核糖核酸酶HI而非核糖核酸酶HII是产生两种msDNA成熟形式所必需的。
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