Magrelli A, Langenkemper K, Dehio C, Schell J, Spena A
Max-Plank-Institut für Züchtungsforschung, Cologne, Germany.
Science. 1994 Dec 23;266(5193):1986-8. doi: 10.1126/science.7528444.
The rolA gene encoded on the Ri plasmid A4 of Agrobacterium rhizogenes is one of the transferred (TL-DNA) genes involved in the pathogenesis of hairy-root disease in plants. The function of the 100-amino acid protein product of rolA is unknown, although its expression causes physiological and developmental alterations in transgenic plants. The rolA gene of A. rhizogenes contains an intron in its untranslated leader region that has features typical of plant pre-messenger RNA introns. Transcription and splicing of the rolA pre-messenger RNA occur in the plant cell.
发根农杆菌Ri质粒A4上编码的rolA基因是参与植物毛状根病发病机制的转移（TL-DNA）基因之一。尽管rolA基因100个氨基酸的蛋白质产物的表达会导致转基因植物发生生理和发育改变，但其功能尚不清楚。发根农杆菌的rolA基因在其非翻译前导区含有一个内含子，该内含子具有植物前体信使RNA内含子的典型特征。rolA前体信使RNA的转录和剪接发生在植物细胞中。
