Hansen G, Larribe M, Vaubert D, Tempé J, Biermann B J, Montoya A L, Chilton M D, Brevet J
Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif Sur Yvette, France.
Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7763-7. doi: 10.1073/pnas.88.17.7763.
This paper presents the map and DNA sequence analysis of pRi8196 transferred DNA (T-DNA) genes encoding root-inducing and mannopine synthesis functions. A canonical 24-base-pair border repeat as well as two "pseudoborders" are present at the functional right T-DNA border. To the left of this border are homologs of the mas1' and mas2' genes of TR pRiA4. Next to these are five open reading frames (ORFs) homologous to ORFs 10-14 of TL of pRiA4. ORFs 10-12 (rolA, rolB, and rolC) are less related to their pRiA4 homologs than are the other large ORFs analyzed here. In contrast to T-DNA genes of pRiA4, pRi8196 T-DNA ORFs 11 and 12 (rolB and rolC) are sufficient to induce hairy roots on carrot disks.
本文展示了编码发根诱导和甘露碱合成功能的发根农杆菌8196（pRi8196）转移DNA（T-DNA）基因的图谱及DNA序列分析。在功能性右T-DNA边界处存在一个典型的24碱基对边界重复序列以及两个“假边界”。在此边界左侧是发根农杆菌pRiA4的mas1'和mas2'基因的同源物。紧挨着这些的是五个与发根农杆菌pRiA4 TL的ORF10 - 14同源的开放阅读框（ORF）。与本文分析的其他大ORF相比，ORF10 - 12（rolA、rolB和rolC）与其pRiA4同源物的相关性较低。与发根农杆菌pRiA4的T-DNA基因不同，发根农杆菌pRi8196的T-DNA的ORF11和12（rolB和rolC）足以在胡萝卜圆片上诱导出毛状根。
