Kurpisz M, Alexander N J
Institute of Human Genetics, Polish Academy of Sciences, Poznan.
Fertil Steril. 1995 Jan;63(1):158-65.
To study the cross-reactions between mouse monoclonal antisperm antibodies and somatic cells or bacteria, to identify the antigenic determinants responsible for such cross-reactions, and to correlate between the antibody function and determinant recognition.
Activities of monoclonal antibodies (mAbs) were characterized by immunosorbent assay of RIA technique; sperm epitopic characterization was performed in lectin-blocking and sugar competitive assays and correlated with functional assays.
Procedures were performed in a university laboratory.
The extensive cross-reactivity between antigenic determinants of sperm, erythrocytes, and bacteria (but not bacterial deglycosylated lipopolysaccharides) was observed. The analytic procedures indicated predominant mAb reactions to carbohydrates such as fucose, galactose, mannose, N-acetylglucosamine and N-acetylgalactosamine. Approximately half of the 30 tested mAbs interfered in the functional assays, that is, sperm agglutination, immobilization, and zona-free penetration.
Sperm carbohydrates seem to induce antibody reactions to common antigenic determinant(s) present on gametes, somatic cells, and infectious agents. Thus molecular mimicry between bacteria and sperm can be a major factor inducing antisperm immunological reactions. Obtained antisperm mAbs, reacting to glycosylated epitopes, presented very strong properties in sperm agglutination and/or immobilization. This did not correlate with inhibiting properties of some antibodies in xenogeneic zona-free penetration test, that is, this assay possibly is not based on oligosaccharide mediation.
研究小鼠单克隆抗精子抗体与体细胞或细菌之间的交叉反应，确定导致此类交叉反应的抗原决定簇，并关联抗体功能与决定簇识别。
采用放射免疫分析技术的免疫吸附试验来表征单克隆抗体（mAb）的活性；通过凝集素阻断和糖竞争试验进行精子表位表征，并与功能试验相关联。
在大学实验室进行实验步骤。
观察到精子、红细胞和细菌（但不包括细菌去糖基化脂多糖）的抗原决定簇之间存在广泛的交叉反应性。分析程序表明，mAb主要与岩藻糖、半乳糖、甘露糖、N-乙酰葡糖胺和N-乙酰半乳糖胺等碳水化合物发生反应。在30种测试的mAb中，约有一半干扰了功能试验，即精子凝集、制动和无透明带穿透。
精子碳水化合物似乎会诱导针对配子、体细胞和感染因子上共同抗原决定簇的抗体反应。因此，细菌与精子之间的分子模拟可能是诱导抗精子免疫反应的主要因素。所获得的与糖基化表位反应的抗精子mAb在精子凝集和/或制动方面表现出非常强的特性。这与某些抗体在异种无透明带穿透试验中的抑制特性无关，也就是说，该试验可能不是基于寡糖介导的。
