Chaudhary R K, Jacobsen H
Laboratory for Viral Hepatitis, Health Canada, Ottawa, Ontario.
J Clin Microbiol. 1994 Oct;32(10):2606-8. doi: 10.1128/jcm.32.10.2606-2608.1994.
We investigated three immunoblot assays (RIBA 3.0 from Chiron, Matrix from Abbott Laboratories, and LiaTek III from Organon Teknika) for the detection of antibody to hepatitis C virus. RIBA 3.0 and Matrix require reactivity to two antigens and LiaTek III requires reactivity to one for a sample to be positive. We tested 80 samples that were positive in repeat enzyme immunoassays by supplemental tests. The results showed that 55, 46, and 28% were reactive by LiaTek III, RIBA 3.0, and Matrix, respectively; 54, 33, and 13% of the samples were indeterminate by Matrix, RIBA 3.0, and LiaTek III, respectively. There were 32, 21, and 16% nonreactive samples by LiaTek III, RIBA 3.0, and Matrix, respectively. Of the samples positive by RIBA 3.0, only 50 and 76% were reactive by Matrix and LiaTek III, respectively. A large number of samples that were indeterminate by RIBA 3.0 were positive by LiaTek III (52%). The core antigen was the most reactive antigen in all three tests (48 to 57%). The NS4 antigen in Matrix (20%) and LiaTek III (16%) was poorly reactive, although it performed better in RIBA 3.0 (45%). The NS5 and E2/NS1 antigens made minor contributions to reactivity. The combinations of the core, NS3, and NS4 antigens produced 77% of the RIBA 3.0 and 100% of the Matrix reactive samples. The results showed a poor correlation among the three tests.
我们研究了三种免疫印迹法（Chiron公司的RIBA 3.0、雅培实验室的Matrix以及奥加农泰尼克公司的LiaTek III）用于检测丙型肝炎病毒抗体。RIBA 3.0和Matrix要求样本对两种抗原呈反应性，而LiaTek III要求对一种抗原呈反应性才能判定样本为阳性。我们通过补充检测对80份在重复酶免疫测定中呈阳性的样本进行了测试。结果显示，LiaTek III、RIBA 3.0和Matrix的反应性样本分别为55%、46%和28%；Matrix、RIBA 3.0和LiaTek III的不确定样本分别为54%、33%和13%。LiaTek III、RIBA 3.0和Matrix的无反应性样本分别为32%、21%和16%。在RIBA 3.0呈阳性的样本中，分别只有50%和76%在Matrix和LiaTek III中呈反应性。大量在RIBA 3.0中不确定的样本在LiaTek III中呈阳性（52%）。核心抗原在所有三项检测中反应性最强（48%至57%）。Matrix（20%）和LiaTek III（16%）中的NS4抗原反应性较差，不过在RIBA 3.0中表现较好（45%）。NS5和E2/NS1抗原对反应性的贡献较小。核心、NS3和NS4抗原的组合产生了77%的RIBA 3.0反应性样本和100%的Matrix反应性样本。结果表明这三项检测之间相关性较差。
