Nübling C M, von Wangenheim G, Staszewski S, Löwer J
Paul-Ehrlich-Institut, Langen, Germany.
J Med Virol. 1994 Sep;44(1):49-53. doi: 10.1002/jmv.1890440110.
Sera of 383 human immunodeficiency virus (HIV)-1-infected individuals from Frankfurt (Main)/Germany were assayed by two hepatitis C virus (HCV) screening tests (Abbott second generation, Ortho second generation). This population showed a prevalence for reactivity with both tests of 20.8% (80/383). Examination of all reactive sera (91/383) by a supplemental assay (Chiron RIBA 2) gave for 46 sera a positive, for 33 sera an indeterminate, and for 12 sera a negative result. Further analysis focussed on these RIBA 2-indeterminate and -negative samples. Analysis of the sera using an in-house Western blot with three different Escherichia coli-expressed HCV proteins revealed that none of the RIBA 2-negative, but 24 of the 33 RIBA 2-indeterminate sera, including 3 of 4 c33c (NS3)-reactive samples, were reactive with a recombinant core protein. Twenty-one of 22 c22-3 (core) indeterminates stained the core antigen in the in-house Western blot and 3 of them in addition a NS5 moiety. HCV-polymerase chain reaction (PCR) was positive for 14 of the 24 RIBA 2-indeterminate sera, but for none of the RIBA 2-negative or Western blot nonreactive samples. Discrepant results between the two screening tests could not be explained by differences in the antigen compositions (i.e., a NS3-NS4 moiety of 111 amino acids present in the Ortho enzyme-linked immunosorbent assay (ELISA), not present in the Abbott or RIBA 2 assays).
采用两种丙型肝炎病毒（HCV）筛查检测方法（雅培第二代、奥索第二代）对来自德国美因河畔法兰克福的383名人类免疫缺陷病毒（HIV）-1感染者的血清进行了检测。该人群中两种检测均呈反应性的患病率为20.8%（80/383）。通过一种补充检测方法（Chiron RIBA 2）对所有反应性血清（91/383）进行检测，结果显示46份血清为阳性，33份血清结果不确定，12份血清为阴性。进一步分析聚焦于这些RIBA 2结果不确定和阴性样本。使用一种内部蛋白质印迹法，用三种不同的大肠杆菌表达的HCV蛋白对血清进行分析，结果显示，RIBA 2阴性的血清中无一与重组核心蛋白发生反应，但33份RIBA 2结果不确定的血清中有24份与重组核心蛋白发生反应，其中包括4份c33c（NS3）反应性样本中的3份。22份c22-3（核心）结果不确定的样本中有21份在内部蛋白质印迹法中与核心抗原发生反应，其中3份还与NS5部分发生反应。HCV聚合酶链反应（PCR）在24份RIBA 2结果不确定的血清中有14份呈阳性，但在RIBA 2阴性或蛋白质印迹法无反应的样本中均为阴性。两种筛查检测结果的差异无法用抗原组成的差异来解释（即奥索酶联免疫吸附测定（ELISA）中存在的111个氨基酸的NS3-NS4部分，在雅培或RIBA 2检测中不存在）。
