Capucci M S, Hoffmann M E, Zdzienicka M Z, Natarajan A T
MGC Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
Mutat Res. 1995 Feb;326(2):227-34. doi: 10.1016/0027-5107(94)00172-2.
The genotoxic effects induced by the monofunctional nitrosourea derivative streptozotocin (STZ) were investigated in Chinese hamster ovary cells, parental (CHO-9) and its mutant hypersensitive to alkylating agents, designated EM-C11. The ability of this compound to induce chromosomal aberrations, cell killing, sister-chromatid exchanges (SCEs) and mutations was evaluated on these two cell lines. The mutant cells were found to be slightly more sensitive to the killing effects of STZ than the parental cell line. EM-C11 cells also showed higher levels of STZ-induced chromosomal aberrations than CHO-9 cells, but appeared to be equally sensitive to induction of SCEs. The frequencies of STZ-induced mutations, measured as resistant Na+/K(+)-ATPase and HPRT mutants, revealed a higher sensitivity of EM-C11 to the mutagenic effects of this compound.
研究了单功能亚硝基脲衍生物链脲佐菌素（STZ）对中国仓鼠卵巢细胞及其对烷化剂敏感的突变体（命名为EM-C11）的遗传毒性作用。在这两种细胞系上评估了该化合物诱导染色体畸变、细胞杀伤、姐妹染色单体交换（SCE）和突变的能力。发现突变细胞对STZ的杀伤作用比亲本细胞系略敏感。EM-C11细胞中STZ诱导的染色体畸变水平也高于CHO-9细胞，但对SCE诱导的敏感性似乎相同。以抗Na+/K(+)-ATP酶和HPRT突变体衡量的STZ诱导突变频率显示，EM-C11对该化合物的诱变作用更敏感。
