López-Aparicio P, Recio M N, Prieto J C, Pérez-Albarsanz M A
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Biosci Rep. 1994 Jun;14(3):131-8. doi: 10.1007/BF01240245.
The influence of lindane (gamma-hexachlorocyclohexane) on fluidity of plasma membranes from rat renal cortical tubules has been investigated. Preincubation with lindane increased membrane fluidity. This effect was accompanied by (i) a decrease in the transport of glucose with regard to the controls and (ii) an inhibition of the beta-adrenergic stimulatory activity upon cyclic AMP accumulation. However, a significant decrease of the membrane fluidity was found when rats were injected with lindane for 12 days. The injection of lindane exerted the opposite effect on the membrane proteins, the glucose transporter and the beta-adrenergic receptor, enhancing the glucose uptake and increasing the isoproterenol-stimulated cycle AMP accumulation. A possible explanation of the difference could involve a resistance to membrane disordering by lindane through a regulatory mechanism that would balance the activity of many lindane-sensitive proteins in insecticide-injected rats.
研究了林丹（γ-六氯环己烷）对大鼠肾皮质肾小管质膜流动性的影响。用林丹预孵育可增加膜流动性。这种效应伴随着：（i）与对照组相比葡萄糖转运减少；（ii）对β-肾上腺素能刺激活性（关于环磷酸腺苷积累）的抑制。然而，当给大鼠注射林丹12天时，发现膜流动性显著降低。注射林丹对膜蛋白、葡萄糖转运体和β-肾上腺素能受体产生相反的作用，增强了葡萄糖摄取并增加了异丙肾上腺素刺激的环磷酸腺苷积累。这种差异的一个可能解释可能涉及通过一种调节机制对林丹引起的膜紊乱产生抗性，该机制将平衡注射杀虫剂的大鼠中许多对林丹敏感的蛋白质的活性。
