Liu P, Shu H, Zhang Y
Department of Hygiene, Xuzhou Medical College.
Zhonghua Liu Xing Bing Xue Za Zhi. 1994 Jun;15(3):179-81.
The feasibility of screening for the antibody to HCV by pooling 10 serum specimens was studied by utilizing the epidemiologic method of single blind random allocation. We changed only the ratio between the pooled serum specimen volume and the serum specimen diluent volume, with their total volume and single serum specimen volume being as much as the volume required by EIA. Other steps were the same as those of EIA. The result showed that taking single serum specimen as the control group, the sensitivity and specificity of pooled serum specimen were 100% and 97.6%, respectively. The false positive rate was 2.4%, Kappa value was 0.95 (P < 0.05). The analysis of profit found that remarkable social and economic benefit will be obtained when serum pooling method is used. These results demonstrate that pooling 10 serum specimens is feasible to screening the antibody to HCV in blood donors by EIA.
采用单盲随机分配的流行病学方法，研究了将10份血清标本混合用于丙型肝炎病毒（HCV）抗体筛查的可行性。我们仅改变了混合血清标本体积与血清标本稀释液体积之间的比例，其总体积和单份血清标本体积与酶免疫分析（EIA）所需体积相同。其他步骤与EIA相同。结果显示，以单份血清标本作为对照组，混合血清标本的敏感性和特异性分别为100%和97.6%。假阳性率为2.4%，Kappa值为0.95（P<0.05）。效益分析发现，采用血清混合法将获得显著的社会和经济效益。这些结果表明，将10份血清标本混合用于通过EIA筛查献血者的HCV抗体是可行的。
