Yamate J, Tajima M, Saitoh T, Shibuya K
Nippon Institute for Biological Science, Tokyo, Japan.
J Comp Pathol. 1994 Oct;111(3):243-57. doi: 10.1016/s0021-9975(05)80003-9.
A subcutaneously transplantable meningeal tumour (MM-KMY) was derived from a spontaneous malignant meningioma which developed in the cerebellar meninges of a female F344 rat. MM-KMY was subjected to 25 serial passages in syngeneic male and female rats. The transplants grew in 8 weeks into a nodule with an average diameter of 5.7 cm and average weight of 125.2 g. MM-KMY possessed large cysts containing fluid and necrotic tissue. Metastases frequently occurred in the lungs of MM-KMY-bearing rats. Histologically, both the original tumour and MM-KMY consisted of round to fusiform neoplastic cells of varying size, with nuclear pleomorphism. Mitotic figures occurred frequently. MM-KMY cells were positive for vimentin. Ultrastructurally, the cells showed desmosome-like structures, interdigitating processes and cytoplasmic intermediate filaments, suggesting an arachnoid cell origin. Abnormal accumulations of hyaline droplets in renal tubular epithelial cells were frequently observed in MM-KMY-bearing rats, suggesting overload of low molecular proteins in the renal tubules. The droplets gave a faint immunoreaction for lysozyme. The relation between the appearance of renal tubular hyaline droplets and the growth of MM-KMY remains to be determined. MM-KMY may prove useful for studying the biological behaviour and morphogenesis of meningeal tumours.
一种可皮下移植的脑膜瘤（MM-KMY）源自一只雌性F344大鼠小脑脑膜中自发形成的恶性脑膜瘤。MM-KMY在同基因的雄性和雌性大鼠中进行了25代连续传代。移植瘤在8周内生长成一个平均直径为5.7厘米、平均重量为125.2克的结节。MM-KMY有含有液体和坏死组织的大囊肿。携带MM-KMY的大鼠肺部经常发生转移。组织学上，原发肿瘤和MM-KMY均由大小不一的圆形至梭形肿瘤细胞组成，具有核多形性。有丝分裂象频繁出现。MM-KMY细胞波形蛋白呈阳性。超微结构上，细胞显示出桥粒样结构、指状突和细胞质中间丝，提示起源于蛛网膜细胞。在携带MM-KMY的大鼠中经常观察到肾小管上皮细胞中透明滴的异常积聚，提示肾小管中低分子蛋白过载。这些滴状物对溶菌酶呈弱阳性免疫反应。肾小管透明滴的出现与MM-KMY生长之间的关系尚待确定。MM-KMY可能对研究脑膜瘤的生物学行为和形态发生有用。
