Glennon M, Smith T, Cormican M, Noone D, Barry T, Maher M, Dawson M, Gilmartin J J, Gannon F
National Diagnostics Centre, BioResearch Ireland, University College Galway.
Tuber Lung Dis. 1994 Oct;75(5):353-60. doi: 10.1016/0962-8479(94)90081-7.
DNA Diagnostics, The National Diagnostics Centre and University College Hospital, Galway, Republic of Ireland.
To investigate the possibility of developing a DNA probe to distinguish the members of the Mycobacterium tuberculosis complex and develop an assay for the detection of M. tuberculosis in sputum.
The ribosomal intergenic spacer regions from the members of the M. tuberculosis complex were sequenced and analysed for the ability of this region to provide sequence diversity for DNA probe development.
The 100% sequence homology in this region precluded the development of a probe to distinguish the individual members of the M. tuberculosis complex. However it was possible to develop a DNA probe that could specifically detect only the members of the M. tuberculosis complex.
A specific DNA probe was developed for the detection of the M. tuberculosis complex and its application to M. tuberculosis detection in sputum was demonstrated.
爱尔兰共和国戈尔韦的国家诊断中心DNA诊断部及大学学院医院。
研究开发一种DNA探针以区分结核分枝杆菌复合群各成员的可能性，并开发一种检测痰液中结核分枝杆菌的检测方法。
对结核分枝杆菌复合群各成员的核糖体基因间隔区进行测序，并分析该区域为DNA探针开发提供序列多样性的能力。
该区域100%的序列同源性使得无法开发出区分结核分枝杆菌复合群各成员的探针。然而，有可能开发出一种只能特异性检测结核分枝杆菌复合群各成员的DNA探针。
开发出了一种用于检测结核分枝杆菌复合群的特异性DNA探针，并证明了其在痰液中结核分枝杆菌检测中的应用。
