Novelli E L, Valente J P, Rodrigues N L
Departamento de Química, Universidade Estadual Paulista, UNESP, Botucatu, São Paulo, Brazil.
Toxicon. 1994 Oct;32(10):1270-4. doi: 10.1016/0041-0101(94)90357-3.
Pollution, industrial solvents, concentrations of metals and other environmental agents are widely related to biochemicals values which are used in disease diagnosis of environmental toxicity. A rat bioassay validated for the identification of toxic effects of eutrophication revealed increased serum activities of amylase, alanine transaminase (ALT) and alkaline phosphatase (ALP) in rats that received algae, filtered water and nickel or cadmium from drinking water. Serum Cu-Zn superoxide dismutase activity decreased from its basal level of 40.8 +/- 2.3 to 26.4 U/mg protein, at 7 days of algae and at 48 hr of nickel and cadmium water ingestion. The observation that lipoperoxide concentration was not altered in rats treated with filtered water, while amylase, ALT and ALP were increased in these rats and in those treated with nickel or cadmium, indicated that pancreatic, hepatic and osteogenic lesions by eutrophication were not related to superoxide radicals, and might be due to a novel toxic environmental agent found in filtered and non-filtered algae water.
污染、工业溶剂、金属浓度及其他环境因素与用于环境毒性疾病诊断的生化指标密切相关。一项经验证可用于识别富营养化毒性作用的大鼠生物测定显示，饮用含有藻类、过滤水以及镍或镉的大鼠血清淀粉酶、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）活性增加。在摄入藻类7天以及摄入镍和镉水48小时后，血清铜锌超氧化物歧化酶活性从其基础水平40.8±2.3降至26.4 U/mg蛋白质。观察发现，饮用过滤水的大鼠脂质过氧化物浓度未改变，而这些大鼠以及饮用镍或镉水的大鼠中淀粉酶、ALT和ALP增加，这表明富营养化引起的胰腺、肝脏和成骨损伤与超氧自由基无关，可能是由于在过滤和未过滤的藻类水中发现的一种新型有毒环境因子所致。
