超氧自由基与环境镍暴露的毒性

Superoxide radical and toxicity of environmental nickel exposure.

作者信息

Novelli E L, Rodrigues N L, Ribas B O

机构信息

Departamento de Química, Universidade Estadual Paulista, UNESP, Botucatu, São Paulo, Brasil.

出版信息

Hum Exp Toxicol. 1995 Mar;14(3):248-51. doi: 10.1177/096032719501400303.

DOI:10.1177/096032719501400303

PMID:7779454

Abstract

Three nickel compounds were tested for pancreatic, hepatic and osteogenic damage in rats by a single i.m. injection Ni++ (7 mg kg-1). The nickel induced biochemical alterations included significantly increased levels of serum alkaline phosphatase in rats with NiS (75%) and NiO (50%). Amylase and aspartate transaminase were also increased, and lipoperoxide was increased in rats with NiO (5.6-fold) and NiS (3.4-fold). No serum changes were observed with NiCl2. Daily injection of Cu-Zn superoxide dismutase (SOD) conjugated with polyethylene glycol prevented the serum level changes, indicating that superoxide radical is an important intermediate in toxicity of nickel insoluble compounds.

摘要

通过单次肌肉注射7mg/kg的Ni++，对三种镍化合物在大鼠体内的胰腺、肝脏和骨生成损伤情况进行了测试。镍诱导的生化改变包括，在注射硫化镍（75%）和氧化镍（50%）的大鼠中，血清碱性磷酸酶水平显著升高。淀粉酶和天冬氨酸转氨酶也升高，在注射氧化镍（5.6倍）和硫化镍（3.4倍）的大鼠中，脂质过氧化物增加。注射氯化镍未观察到血清变化。每日注射与聚乙二醇结合的铜锌超氧化物歧化酶（SOD）可防止血清水平变化，这表明超氧自由基是镍不溶性化合物毒性的重要中间产物。