Ruck P, Xiao J C, Kaiserling E
Institute of Pathology, University of Tübingen, Germany.
Arch Pathol Lab Med. 1995 Feb;119(2):173-8.
The reactivity of sinusoids in hepatocellular carcinoma (HCC), focal nodular hyperplasia, and nonneoplastic liver tissue with various endothelial markers was investigated to detect any differences that might be of diagnostic relevance. The lectin UEA-1 antibody BMA 120, and antibodies against von Willebrand's factor, CD31, and CD34 were used. KP1 was employed to detect Kupffer cells. In the normal liver there was only focal staining of sinusoidal endothelium in the vicinity of the portal tracts with all of the endothelial markers applied. In the cirrhotic liver a slightly greater number of sinusoids (mainly in the vicinity of the fibrous septa) stained with UEA-1 and, although to a lesser extent, with anti-von Willebrand's factor and anti-CD31. A slight increase in staining for CD34 was seen in only 1 of the 11 specimens of cirrhotic liver. In focal nodular hyperplasia, there was increased staining of sinusoids with all of the markers investigated; staining was confined mainly to the periphery of the nodules. HCC exhibited the most obvious differences in numbers of stained sinusoids and staining intensity in comparison with both normal and cirrhotic liver. UEA-1 and anti-CD34 stained large numbers of sinusoids in virtually all of the HCC investigated; UEA-1 stained a slightly greater number of sinusoids and did so with slightly greater intensity. BMA 120 and the antibodies against von Willebrand's factor and CD31 stained a smaller number of sinusoids and did so with lower intensity; they failed to stain sinusoids in some of the tumors. Because staining of the sinusoids in cirrhotic liver was minimal with anti-CD34, this antibody proved to be the best of all the markers investigated for distinguishing highly differentiated HCC from nonneoplastic liver tissue. It seems possible that the increase in immunoreactivity of sinusoids in HCC with anti-CD, unlike that with Uea-1, anti-von Willebrand's factor, and anti-CD31, is not an expression of capillarization, but rather of angiogenesis.
研究肝细胞癌（HCC）、局灶性结节性增生及非肿瘤性肝组织中肝血窦与各种内皮标志物的反应性，以检测可能具有诊断意义的差异。使用了凝集素UEA - 1抗体BMA 120、抗血管性血友病因子抗体、抗CD31抗体和抗CD34抗体。采用KP1检测库普弗细胞。在正常肝脏中，应用的所有内皮标志物仅在门静脉周围的肝血窦内皮有局灶性染色。在肝硬化肝脏中，有稍多数量的肝血窦（主要在纤维间隔附近）被UEA - 1染色，抗血管性血友病因子和抗CD31染色程度稍低。在11例肝硬化肝脏标本中，仅有1例CD34染色略有增加。在局灶性结节性增生中，所有研究的标志物对肝血窦的染色均增加；染色主要局限于结节周边。与正常肝脏和肝硬化肝脏相比，HCC在肝血窦染色数量和染色强度上表现出最明显的差异。在几乎所有研究的HCC中，UEA - 1和抗CD34染色大量肝血窦；UEA - 1染色的肝血窦数量略多，强度也略高。BMA 120、抗血管性血友病因子抗体和抗CD31染色的肝血窦数量较少，强度较低；在一些肿瘤中它们未能使肝血窦染色。由于抗CD34对肝硬化肝脏肝血窦的染色极少，该抗体被证明是所有研究标志物中区分高分化HCC与非肿瘤性肝组织的最佳标志物。与UEA - 1、抗血管性血友病因子和抗CD31不同，HCC中肝血窦与抗CD免疫反应性的增加似乎不是毛细血管化的表现，而是血管生成的表现。
