Manley M S, Young S J, Groves P M
Department of Psychiatry, University of California, San Diego 92093-0603.
J Chem Neuroanat. 1994 Aug;7(3):191-201. doi: 10.1016/0891-0618(94)90029-9.
The mammalian striatum may be divided into a striosomal compartment and a surrounding matrix region. We have examined the distribution of leucine enkephalin (LENK) and substance P (SP) immunoreactivity in relation to striosomes defined by calbindin-D (CABD) staining in alternate 70 microns serial sections from the human caudate nucleus. The distribution of LENK immunoreactivity showed a transition from dorsal to ventral striatum: dorsally, LENK-rich patches were present in a lightly stained matrix; mid-ventrally, annular patches of LENK staining with a lighter core were seen. These patches corresponded to striosomal regions defined by CABD-poor zones. In contrast, in the ventral caudate and nucleus accumbens, LENK-poor zones matched CABD-defined striosomes. CABD staining in the matrix was intense in the dorsal caudate, diminishing ventrally. SP-rich zones in dorsal caudate and SP-poor areas in the mid-ventral region overlapped striosomes. In the ventromedial sector, the SP staining pattern was complex and did not consistently correlate with striosomes. Computer-assisted three-dimensional reconstruction of the striosomal system in the human, based on regions of either high LENK or low CABD immunoreactivity, revealed the existence of considerable long-range order. Patches appeared aligned over several millimeters to form long, horizontal structures in the caudate nucleus, with occasional orthogonal interconnecting crossbridges. Our results are in accord with previous work in the human and in other species. These three-dimensional networks are strikingly similar across individuals and may relate to the segregation of and interactions between striatal circuits.
哺乳动物的纹状体可分为纹体区和周围的基质区。我们在人尾状核每隔70微米的连续切片中，研究了亮氨酸脑啡肽（LENK）和P物质（SP）免疫反应性的分布与由钙结合蛋白-D（CABD）染色定义的纹体的关系。LENK免疫反应性的分布显示出从背侧纹状体到腹侧纹状体的转变：在背侧，富含LENK的斑块存在于轻度染色的基质中；在腹中部，可见LENK染色的环形斑块，其核心较淡。这些斑块对应于由CABD缺乏区定义的纹体区域。相反，在腹侧尾状核和伏隔核中，LENK缺乏区与CABD定义的纹体相匹配。基质中的CABD染色在背侧尾状核中强烈，在腹侧逐渐减弱。背侧尾状核中的富含SP的区域和腹中部区域中的缺乏SP的区域与纹体重叠。在腹内侧区，SP染色模式复杂，与纹体没有一致的相关性。基于高LENK或低CABD免疫反应性区域对人类纹体系统进行计算机辅助三维重建，揭示了相当长距离的有序性的存在。斑块在几毫米的范围内排列，在尾状核中形成长的水平结构，偶尔有正交的相互连接的交叉桥。我们的结果与之前在人类和其他物种中的研究结果一致。这些三维网络在个体之间非常相似，可能与纹状体回路的分离和相互作用有关。
