Solomons N W, Schneider R E, García Ibáñez R, Pineda O, Viteri F E, Lizarralde E, Schoeller D, Klein P, Rosenberg I H, Calloway D
Arch Latinoam Nutr. 1978 Sep;28(3):301-17.
Tests based on the analysis of the gaseous components of expired air have been developed to study intestinal absorption and intermediary metabolism of various nutrients. This paper reviews the breath-analysis tests based on the measurement of CH4, H2, and isotopically-labelled CO2 for studying the intestinal absorption of carbohydrates, fats, and bile salts, and intrahepatic metabolism. New technology employing mass spectrometry allows the use of the stable isotope, carbon-13, instead of the radioactive isotope, carbon-14, for CO2 breath tests. The nutritional application of the breath-analysis tests is discussed, and the advantages of the non-radioactive, non-invasive procedures, especially for use in children and pregnant women in whom standard investigational methods represent a discomfort or a radioactive hazard, are emphasized.
基于对呼出气体中气态成分分析的检测方法已被开发出来,用于研究各种营养素的肠道吸收和中间代谢。本文综述了基于测量CH4、H2和同位素标记的CO2的呼吸分析检测方法,以研究碳水化合物、脂肪和胆汁盐的肠道吸收以及肝内代谢。采用质谱的新技术允许在CO2呼气试验中使用稳定同位素碳-13,而不是放射性同位素碳-14。讨论了呼吸分析检测方法在营养方面的应用,并强调了非放射性、非侵入性检测方法的优点,特别是对于儿童和孕妇,在这些人群中标准的研究方法会带来不适或放射性危害。