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[基于呼出气分析的检测方法在营养研究中的应用]

[Use of tests based on the analysis of expired air in nutritional studies].

作者信息

Solomons N W, Schneider R E, García Ibáñez R, Pineda O, Viteri F E, Lizarralde E, Schoeller D, Klein P, Rosenberg I H, Calloway D

出版信息

Arch Latinoam Nutr. 1978 Sep;28(3):301-17.

PMID:753181
Abstract

Tests based on the analysis of the gaseous components of expired air have been developed to study intestinal absorption and intermediary metabolism of various nutrients. This paper reviews the breath-analysis tests based on the measurement of CH4, H2, and isotopically-labelled CO2 for studying the intestinal absorption of carbohydrates, fats, and bile salts, and intrahepatic metabolism. New technology employing mass spectrometry allows the use of the stable isotope, carbon-13, instead of the radioactive isotope, carbon-14, for CO2 breath tests. The nutritional application of the breath-analysis tests is discussed, and the advantages of the non-radioactive, non-invasive procedures, especially for use in children and pregnant women in whom standard investigational methods represent a discomfort or a radioactive hazard, are emphasized.

摘要

基于对呼出气体中气态成分分析的检测方法已被开发出来,用于研究各种营养素的肠道吸收和中间代谢。本文综述了基于测量CH4、H2和同位素标记的CO2的呼吸分析检测方法,以研究碳水化合物、脂肪和胆汁盐的肠道吸收以及肝内代谢。采用质谱的新技术允许在CO2呼气试验中使用稳定同位素碳-13,而不是放射性同位素碳-14。讨论了呼吸分析检测方法在营养方面的应用,并强调了非放射性、非侵入性检测方法的优点,特别是对于儿童和孕妇,在这些人群中标准的研究方法会带来不适或放射性危害。

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