Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay.
作者信息
Walmsley M E, Patient R K
机构信息
Developmental Biology Research Centre, King's College, University of London, UK.
出版信息
Mol Biotechnol. 1994 Jun;1(3):265-75. doi: 10.1007/BF02921694.
PMID:7532103
Abstract
A method for determining the beginning and end of gene transcripts along with the position of intron/exon boundaries by S1 nuclease protection is described. Probe strategies and hybridization conditions are considered in detail. Under conditions of probe excess the method is quantitative.
摘要
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