Matsuoka M, Matsubara M, Inoue J, Kakehi M, Imanaka T
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
Curr Genet. 1994 Nov-Dec;26(5-6):382-9. doi: 10.1007/BF00309923.
Mitochondrial gene organization was studied in a dimorphic yeast, Yarrowia lipoltica. The gene order in a sequenced 6.6-kilobase region closely resembles that of the human mitochondrial genome in that ATP synthase subunit 8 and 6 genes are followed by genes for cytochrome c oxidase subunit 3 (which contains an intron), NADH-ubiquinone oxidoreductase subunit 4, and ATP synthase subunit 9. This region also contains tRNA genes decoding AUA, UGA, CUN and CCN codons, suggesting a unique mitochondrial translation. All the above genes are transcribed from the same DNA strand into multigenic RNAs, starting from a nonanucleotide sequence, 5'-ATATAAATA-3', similar to other yeast mitochondrial promoters.
在双态酵母解脂耶氏酵母中研究了线粒体基因组织。在一个测序的6.6千碱基区域中的基因顺序与人类线粒体基因组的基因顺序非常相似，即ATP合酶亚基8和6基因之后是细胞色素c氧化酶亚基3（含有一个内含子）、NADH-泛醌氧化还原酶亚基4和ATP合酶亚基9的基因。该区域还包含解码AUA、UGA、CUN和CCN密码子的tRNA基因，表明存在独特的线粒体翻译。上述所有基因均从同一DNA链转录成多基因RNA，起始于一个九核苷酸序列5'-ATATAAATA-3'，类似于其他酵母线粒体启动子。
