Ichikawa T, Kuwaki T, Tachibana K, Yanagida M, Matsuki S
Pharmaceutical Development Laboratory, Kirin Brewery Co., Ltd., Japan.
Exp Hematol. 1995 Mar;23(3):192-5.
We have developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) to measure granulocyte colony-stimulating factor (G-CSF) in human plasma. This ELISA employs a combination of a mouse monoclonal antibody (MAb) as the first antibody and an affinity-purified sheep polyclonal antibody conjugated with beta-D-galactosidase as the second antibody. The coefficients of intra- and interassay variations were 2.2 to 3.6% and 8.3 to 10.2%, respectively. The assay had no cross-reactivity with four other human cytokines. Plasma G-CSF levels in healthy volunteers could easily be measured because the detection limit was 0.5 pg/mL. The mean plasma G-CSF concentration in 57 healthy volunteers (aged 19 to 47; 27 males and 30 females) was 10.5 +/- 4.5 pg/mL standard deviation [SD]). There was no statistically significant relationship between plasma G-CSF concentration and absolute neutrophil counts in those healthy volunteers (r = 0.259; p = 0.052).
我们开发了一种灵敏的夹心酶联免疫吸附测定法（ELISA），用于检测人血浆中的粒细胞集落刺激因子（G-CSF）。该ELISA采用鼠单克隆抗体（MAb）作为第一抗体，以及与β-D-半乳糖苷酶偶联的亲和纯化羊多克隆抗体作为第二抗体。批内和批间变异系数分别为2.2%至3.6%和8.3%至10.2%。该测定法与其他四种人类细胞因子无交叉反应。由于检测限为0.5 pg/mL，健康志愿者的血浆G-CSF水平很容易测定。57名健康志愿者（年龄19至47岁；男性27名，女性30名）的血浆G-CSF平均浓度为10.5±4.5 pg/mL（标准差[SD]）。在这些健康志愿者中，血浆G-CSF浓度与绝对中性粒细胞计数之间无统计学显著关系（r = 0.259；p = 0.052）。
