Niu Z, Ge Z, Fang J
PUMC Hospital, CAMS, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1994 Oct;16(5):370-3.
By adding monoclonal antibodies from BALB/c mice to the granulocyte colony-stimulating factor (G-CSF), we established a sandwich ELISA for detecting G-CSF levels in human serum samples. G-CSF was measured in 157 normal serum samples and in 153 serum samples from patients with no clinical manifestation of bacterial infection and in 269 serum samples from patients with clinical diagnosis of bacterial infection and in 31 serum samples from patients with positive bacterial infection according to cell culture. It was found that the positive rates of G-CSF were zero in normal serum samples, only 6.5% in patients with no clinical manifestation of bacterial infection, 89.2% in patients with clinical diagnosis of bacterial infection and 100% in patients with bacterial culture positive for infection. Our results reveal that the sandwich ELISA for detection of G-CSF levels in human serum samples may be useful for diagnosing patients with bacterial infection and for clinically guiding the rational use of antibiotics.
通过将来自BALB/c小鼠的单克隆抗体添加到粒细胞集落刺激因子（G-CSF）中，我们建立了一种夹心酶联免疫吸附测定法（ELISA）来检测人血清样本中的G-CSF水平。对157份正常血清样本、153份无细菌感染临床表现患者的血清样本、269份临床诊断为细菌感染患者的血清样本以及31份根据细胞培养细菌感染呈阳性患者的血清样本进行了G-CSF检测。结果发现，正常血清样本中G-CSF的阳性率为零，无细菌感染临床表现的患者中仅为6.5%，临床诊断为细菌感染的患者中为89.2%，细菌培养感染呈阳性的患者中为100%。我们的结果表明，用于检测人血清样本中G-CSF水平的夹心ELISA可能有助于诊断细菌感染患者，并在临床上指导抗生素的合理使用。
